PMID- 11172495
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20191111
IS  - 1084-8592 (Print)
IS  - 1084-8592 (Linking)
VI  - 5
IP  - 4
DP  - 2000 Dec
TI  - The role of fluorescence in situ hybridization technologies in molecular 
      diagnostics and disease management.
PG  - 309-19
AB  - Large genomic changes, such as aneuploidy, deletions, and other chromosomal 
      rearrangements, have long been associated with pregnancy loss, congenital 
      abnormalities, and malignancy. These genomic changes are quantitative, 
      unambiguous, and fundamental in the transition of normal cells to abnormal ones. 
      Detection of these large genetic changes has an increasingly important role in 
      determining patient diagnosis and care, including therapeutic selection. We have 
      developed two major product platforms that assess genomic changes at various 
      levels of resolution. Fluorescence in situ hybridization (FISH) techniques and 
      the related technology of array-based comparative genomic hybridization (CGH) 
      allow detection of genesized or larger alterations in the genome. FISH is a 
      robust DNA probe technology that can measure both balanced and unbalanced genomic 
      changes on a cell-by-cell basis. In most instances, it is not dependent on 
      metaphase chromosomes, and it is widely used in clinical diagnostics. Array-based 
      CGH has much greater multiplexing capabilities than FISH. This technology has the 
      potential to examine many regions of the genome simultaneously for changes in DNA 
      copy number and identify complex patterns of gains and losses within the genome. 
      In this article, we review several of the current medical applications of FISH 
      and discuss such advanced techniques as CGH and array-based CGH.
FAU - King, W
AU  - King W
AD  - Vysis, Inc, 3199 Woodcreek Dr., Downers Grove, IL 60515, USA.
FAU - Proffitt, J
AU  - Proffitt J
FAU - Morrison, L
AU  - Morrison L
FAU - Piper, J
AU  - Piper J
FAU - Lane, D
AU  - Lane D
FAU - Seelig, S
AU  - Seelig S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Mol Diagn
JT  - Molecular diagnosis : a journal devoted to the understanding of human disease 
      through the clinical application of molecular biology
JID - 9614965
SB  - IM
MH  - Databases, Factual
MH  - *Disease Management
MH  - Genetic Testing/*methods/trends
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods/trends
MH  - Internet
MH  - Oligonucleotide Array Sequence Analysis/methods/trends
RF  - 53
EDAT- 2001/02/15 11:00
MHDA- 2001/05/01 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - S1084859200840497 [pii]
AID - 10.1007/BF03262092 [doi]
PST - ppublish
SO  - Mol Diagn. 2000 Dec;5(4):309-19. doi: 10.1007/BF03262092.