PMID- 11172587
OWN - NLM
STAT- MEDLINE
DCOM- 20010524
LR  - 20190605
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 18
IP  - 2
DP  - 2001 Feb
TI  - Evaluation of myc and chromosome 8 copy number in colorectal cancer using 
      interphase cytogenetics.
PG  - 233-9
AB  - To reveal the significance of genetic abnormalities of the c-myc gene, 56 
      colorectal tumors (43 colorectal carcinomas, 5 recurrent or metastatic tumors, 
      and 8 adenomatous polyps) were analyzed using fluorescence in situ hybridization 
      (FISH). Two probes specific for c-myc and the chromosome 8 centromere were used 
      for dual color FISH. In each case, 100-200 nuclei were observed for signals from 
      the probes. The percent of nuclei with c-myc amplification (PMA) was defined as 
      the proportion of nuclei representing the ratio of c-myc/chromosome 8 >1.0, and 
      the percent of nuclei with the greater number of c-myc (PGNM) was defined as the 
      proportion of nuclei representing the ratio of c-myc/chromosome 8 > or =2.0. Low 
      level amplification was defined as a case with PMA > or =10% and PGNM <10%. High 
      level amplification was defined as a case with PGNM > or =10%. While adenomatous 
      polyps and in situ carcinomas showed no c-myc amplification, the low level 
      amplification and high level amplification of c-myc were observed in 48.8% 
      (21/43) and 20.9% (9/43) of primary colorectal carcinomas. In addition, the group 
      including cases of stage IIIb and IV exhibited significantly higher average copy 
      numbers of c-myc (CN-myc), PMA and PGNM than the other group of earlier stages. 
      FISH was thought a useful cytogenetic method to detect genetic abnormalities in 
      solid tumors. It was shown that the c-myc gene amplification identified using 
      FISH was associated with the aggressiveness of colorectal carcinoma.
FAU - Obara, K
AU  - Obara K
AD  - Department of Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 
      113-8608, Japan. obara@gd5.so-net.ne.jp
FAU - Yokoyama, M
AU  - Yokoyama M
FAU - Asano, G
AU  - Asano G
FAU - Tanaka, S
AU  - Tanaka S
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Colorectal Neoplasms/*genetics
MH  - Gene Amplification/*genetics
MH  - Genes, myc/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Middle Aged
EDAT- 2001/02/15 11:00
MHDA- 2001/05/26 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/05/26 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.3892/ijo.18.2.233 [doi]
PST - ppublish
SO  - Int J Oncol. 2001 Feb;18(2):233-9. doi: 10.3892/ijo.18.2.233.