PMID- 11172616
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20190605
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 7
IP  - 2
DP  - 2001 Feb
TI  - Evaluation of antigenicity of germinated barley foodstuff for the treatment of 
      ulcerative colitis in a chronic murine colitis model.
PG  - 143-7
AB  - Germinated barley foodstuff (GBF) contains insoluble protein and dietary fiber, 
      and has the potential to attenuate diarrhea and colonic mucosal damage in 
      colitis. Since GBF contains a poorly digested protein fraction, this protein may 
      be transferred to and absorbed in the colon. It may therefore be possible to 
      detect the GBF antigen in colitis patients with dysfunctional colonic mucosal 
      barrier defense. In this study, the antigenic potency of GBF was examined in vivo 
      and in vitro. Using the AOAC method, the indigestible fraction of GBF (dietary 
      fiber) was obtained, and the poorly digested protein fraction in GBF was 
      determined. Using Sprague Dawley rats with chronic colitis induced by dextran 
      sulfate sodium (DSS, 2.5% in diet), a GBF and control diet were administered, and 
      total and specific serum immunoglobulin E (IgE) against intestinal contents and 
      the soluble GBF protein were determined. In addition, reactivity between serum 
      and intestinal content were examined by gel electrophoresis mobility shift assay 
      (EMSA). GBF showed relatively low protein digestibility (47%) because of its low 
      solubility in neutral pH. Total serum IgE in both dietary groups was not 
      significantly different, and specific IgE antibodies against intestinal contents 
      and the soluble GBF protein were not significantly different. In addition, 
      supershifts were not observed in either dietary group by EMSA. The possible 
      antigenicity of oral GBF was considered to be low in this colitis model.
FAU - Kanauchi, O
AU  - Kanauchi O
AD  - Nutrient Food and Feed Division, Kirin Brewery Co. Ltd., 10-1-2 Shinkawa Chuo-ku, 
      Tokyo 104-8288, Japan. kanauchio@kirin.co.jp
FAU - Serizawa, I
AU  - Serizawa I
FAU - Matsumura, T
AU  - Matsumura T
FAU - Fukuda, Y
AU  - Fukuda Y
FAU - Satomi, M
AU  - Satomi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Antibodies)
RN  - 0 (Antigens)
RN  - 0 (Dietary Fiber)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Antibodies/blood
MH  - Antigens/immunology/therapeutic use
MH  - Body Weight
MH  - Chronic Disease
MH  - Colitis/chemically induced/diet therapy/therapy
MH  - Colitis, Ulcerative/diet therapy/*therapy
MH  - Dextran Sulfate
MH  - Dietary Fiber/analysis
MH  - Digestion
MH  - Disease Models, Animal
MH  - Germination/immunology
MH  - Hordeum/*immunology/therapeutic use
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Phytotherapy
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2001/02/15 11:00
MHDA- 2001/06/22 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.3892/ijmm.7.2.143 [doi]
PST - ppublish
SO  - Int J Mol Med. 2001 Feb;7(2):143-7. doi: 10.3892/ijmm.7.2.143.