PMID- 11172902
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 124
IP  - 2
DP  - 2001 Jan 15
TI  - Loss of the short arm of the Y chromosome in human prostate carcinoma.
PG  - 122-6
AB  - A change in Y chromosome number is one of the many cytogenetic abnormalities 
      reported in human prostate tumors. However, reports in the literature have varied 
      regarding the frequency of Y loss or gain and the significance of Y aneusomy with 
      respect to the biology of the disease. We have conducted an analysis of the Y 
      chromosome in malignant and benign hyperplastic human prostate epithelium in 
      order to determine whether regional Y loss occurs in prostate cancer. To 
      accomplish this we performed dual-color fluorescence in situ hybridization (FISH) 
      on serial sections of paraffin-embedded prostate tumor tissues using either a Yp 
      (SRY), Ycen (alpha-satellite) or Yq (satellite 3) probe, and an Xcen 
      (alpha-satellite) probe that served as a control for hybridization and nuclear 
      truncation. The results of our FISH analysis demonstrated loss of Yp in the 
      malignant epithelium of 14/40 (35%) prostate tumor sections examined. We also 
      found loss of Yq in 4/40 (10%) of the samples, with one of these exhibiting 
      accompanying Yp loss. The remaining samples, 23/40 (58%), retained both Yp and Yq 
      markers, with no evidence of either Ycen loss or Y gain in any of the tumor 
      samples examined. In addition, Y loss was detected in the benign hyperplastic 
      regions in nearly one-half of the tissue sections that exhibited Y loss in the 
      malignant epithelium. These results demonstrate that regional chromosome Y loss 
      occurs in prostate cancer, that loss of Yp is the most frequent event, and 
      suggest that this loss may in some cases be a precursor to prostate malignancy.
FAU - Jordan, J J
AU  - Jordan JJ
AD  - Department of Radiation Oncology, Fox Chase Cancer Center, 7701 Burholme Avenue, 
      Philadelphia, PA, USA.
FAU - Hanlon, A L
AU  - Hanlon AL
FAU - Al-Saleem, T I
AU  - Al-Saleem TI
FAU - Greenberg, R E
AU  - Greenberg RE
FAU - Tricoli, J V
AU  - Tricoli JV
LA  - eng
GR  - R01-CA6130/CA/NCI NIH HHS/United States
GR  - UO1-CA5189/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (SRY protein, human)
RN  - 0 (Sex-Determining Region Y Protein)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - *Chromosome Deletion
MH  - DNA Probes
MH  - DNA-Binding Proteins
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - *Nuclear Proteins
MH  - Prostatic Hyperplasia/genetics/pathology
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Sex-Determining Region Y Protein
MH  - *Transcription Factors
MH  - *Y Chromosome
EDAT- 2001/02/15 11:00
MHDA- 2001/03/27 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - S0165-4608(00)00340-X [pii]
AID - 10.1016/s0165-4608(00)00340-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Jan 15;124(2):122-6. doi: 
      10.1016/s0165-4608(00)00340-x.