PMID- 11172934
OWN - NLM
STAT- MEDLINE
DCOM- 20010510
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 300
IP  - 1
DP  - 2001 Mar 2
TI  - Distribution of activated caspase-3 in relation with apoptosis in human malignant 
      gliomas.
PG  - 37-40
AB  - Activated caspase-3has been immunohistochemically studied in 30glioblastomas. Its 
      distribution has been compared with that of apoptotic nuclei demonstrated by 
      terminal dUTP nick-end labeling (TUNEL) and morphology. The best procedure for 
      the demonstration of caspase-3 requires formalin fixation, followed by Carnoy 
      fixation, with microwave irradiation. The number of positive cells is lower than 
      that of apoptotic nuclei shown by TUNEL technique, especially in perinecrotic 
      pseudo-palisadings, and there are also qualitative variations. Positive staining 
      occurs in nuclei, cytoplasms or in both cell compartments. The interpretation of 
      Caspase-3 positive staining is based on its crucial position in the final pathway 
      to apoptosis and on the mechanisms by which it cleaves cytoplasmic and nuclear 
      proteins among which inhibitory/caspase-activated DNase system is included.
FAU - Schiffer, D
AU  - Schiffer D
AD  - Department of Neuroscience, University of Turin, Via Cherasco, 15, 10126, Turin, 
      Italy. schiffer@medfarm.unito.it
FAU - Fiano, V
AU  - Fiano V
FAU - Chiado-Piat, L
AU  - Chiado-Piat L
FAU - Mortara, P
AU  - Mortara P
FAU - Richiardi, P
AU  - Richiardi P
FAU - Cavalla, P
AU  - Cavalla P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Apoptosis/*physiology
MH  - Brain Neoplasms/*enzymology
MH  - Caspase 3
MH  - Caspases/*metabolism
MH  - Enzyme Activation
MH  - Glioma/*enzymology
MH  - Humans
MH  - In Situ Nick-End Labeling
EDAT- 2001/02/15 11:00
MHDA- 2001/05/22 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/05/22 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - S0304394001015464 [pii]
AID - 10.1016/s0304-3940(01)01546-4 [doi]
PST - ppublish
SO  - Neurosci Lett. 2001 Mar 2;300(1):37-40. doi: 10.1016/s0304-3940(01)01546-4.