PMID- 11173332
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20191104
IS  - 0916-4804 (Print)
IS  - 0916-4804 (Linking)
VI  - 42
IP  - 1
DP  - 2001
TI  - Detection of gp43 and ITS1-5.8S-ITS2 ribosomal RNA genes of Paracoccidioides 
      brasiliensis in paraffin-embedded tissue.
PG  - 23-7
AB  - Paracoccidioidomycosis (PCM) is a deep mycosis caused by the thermo-dependent 
      dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American 
      countries. An increase in PCM has been reported in recent years and the disease 
      is now recognized as one of the imported fungal infections in Japan. To date, 
      more than 15 cases of PCM have been reported in our country, and five of them 
      were diagnosed by clinical and histopathological findings without mycological 
      study. We applied 2 nested polymerase chain reaction (PCR) amplification methods 
      for detecting P. brasiliensis genes from paraffin-embedded tissue specimens. 
      Successfully amplified were: a 473 base pairs fragment of gp43 gene of P. 
      brasiliensis (located from 741st to 1,213rd base), and a 418 base pairs fragment 
      of 5.8S ribosomal RNA gene of P. brasilienisis which included internal 
      transcribed spacers (ITS) 1 and 2 (located from 131st at ITS1 to 195th at ITS2) 
      in paraffin-embedded murine tissues infected with P. brasiliensis yeast cells. 
      The authenticity of the PCR products was confirmed by nucleotide sequence 
      analysis. These results indicate that the two nested PCR methods may be useful 
      for diagnosis of PCM.
FAU - Sano, A
AU  - Sano A
AD  - Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 
      1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
FAU - Yokoyama, K
AU  - Yokoyama K
FAU - Tamura, M
AU  - Tamura M
FAU - Mikami, Y
AU  - Mikami Y
FAU - Takahashi, I
AU  - Takahashi I
FAU - Fukushima, K
AU  - Fukushima K
FAU - Miyaji, M
AU  - Miyaji M
FAU - Nishimura, K
AU  - Nishimura K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Nihon Ishinkin Gakkai Zasshi
JT  - Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology
JID - 9425640
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Oligosaccharides)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Ribosomal, 5.8S)
SB  - IM
MH  - Animals
MH  - *Antigens, Fungal
MH  - *Fungal Proteins
MH  - *Genes, Fungal
MH  - Glycoproteins/analysis/*genetics
MH  - Humans
MH  - Mice
MH  - Oligosaccharides/analysis/*genetics
MH  - Paracoccidioides/*genetics/isolation & purification
MH  - Paracoccidioidomycosis/*diagnosis/microbiology
MH  - Paraffin Embedding
MH  - Polymerase Chain Reaction
MH  - RNA, Fungal/*analysis
MH  - RNA, Ribosomal, 5.8S/*analysis
EDAT- 2001/02/15 11:00
MHDA- 2001/07/20 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.3314/jjmm.42.23 [doi]
PST - ppublish
SO  - Nihon Ishinkin Gakkai Zasshi. 2001;42(1):23-7. doi: 10.3314/jjmm.42.23.