PMID- 11175666
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20190915
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 155
IP  - 2
DP  - 2001 Feb
TI  - Spatial distribution of selected genetic loci in nuclei of human leukemia cells 
      after irradiation.
PG  - 311-9
AB  - Fluorescence in situ hybridization (FISH) combined with high-resolution cytometry 
      was used to determine the topographic characteristics of the centromeric 
      heterochromatin (of the chromosomes 6, 8, 9, 17) and the tumor suppressor gene 
      TP53 (which is located on chromosome 17) in cells of the human leukemia cell 
      lines ML-1 and U937. Analysis was performed on cells that were either untreated 
      or irradiated with gamma rays and incubated for different intervals after 
      exposure. Compared to untreated cells, homologous centromeres and the TP53 genes 
      were found closer to each other and also closer to the nuclear center 2 h after 
      irradiation. The spatial relationship between genetic elements returned to that 
      of the unirradiated controls during the next 2-3 h. Statistical evaluation of our 
      experimental results shows that homologous centromeres and the homologous genes 
      are positioned closer to each other 2 h after irradiation because they are 
      localized closer to the center of the nucleus (probably due to more pronounced 
      decondensation of the chromatin related to repair). This radial movement of 
      genetic loci, however, is not connected with repair of DSBs by processes 
      involving homologous recombination, because the angular distribution of 
      homologous sequences remains random after irradiation.
FAU - Kozubek, S
AU  - Kozubek S
AD  - Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 
      135, 612 65 Brno, Czech Republic.
FAU - Bartova, E
AU  - Bartova E
FAU - Kozubek, M
AU  - Kozubek M
FAU - Lukasova, E
AU  - Lukasova E
FAU - Cafourkova, A
AU  - Cafourkova A
FAU - Koutna, I
AU  - Koutna I
FAU - Skalnikova, M
AU  - Skalnikova M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Cell Nucleus/chemistry/radiation effects/*ultrastructure
MH  - Centromere/chemistry/radiation effects/ultrastructure
MH  - Chromosomes, Human/chemistry/radiation effects/*ultrastructure
MH  - DNA Damage
MH  - DNA Repair
MH  - Gamma Rays
MH  - Genes/*radiation effects
MH  - Heterochromatin/chemistry/radiation effects/ultrastructure
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia/genetics/*pathology
MH  - Neoplastic Stem Cells/chemistry/radiation effects/*ultrastructure
MH  - Recombination, Genetic
MH  - U937 Cells/chemistry/radiation effects/*ultrastructure
EDAT- 2001/02/15 11:00
MHDA- 2001/04/03 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1667/0033-7587(2001)155[0311:sdosgl]2.0.co;2 [doi]
PST - ppublish
SO  - Radiat Res. 2001 Feb;155(2):311-9. doi: 
      10.1667/0033-7587(2001)155[0311:sdosgl]2.0.co;2.