PMID- 11177575
OWN - NLM
STAT- MEDLINE
DCOM- 20010426
LR  - 20171116
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 21
IP  - 1
DP  - 2001 Jan
TI  - Interleukin-18 production following murine cardiac transplantation: correlation 
      with histologic rejection and the induction of INF-gamma.
PG  - 1-9
AB  - Interleukin-18 (IL-18) and IL-12 have been shown to play an important role in the 
      induction of interferon-gamma (IFN-gamma). IFN-gamma induces the proliferation of 
      T cells and natural killer (NK) cells and augments the Th1 immune cascade. The 
      role of IL-18 and IL-12 in the induction of IFN-gamma following allogeneic heart 
      transplantation has not been described. We sought to characterize the IL-12 and 
      IL-18 response to murine allogeneic heart transplantation, particularly with 
      respect to IFN-gamma production and histologic transplant rejection. Forty-eight 
      heterotopic heart transplants were performed in two groups of mice: syngeneic 
      C3H/HeN to C3H/HeN mice and allogeneic BALB/C to C3H/HeN mice. Transplants were 
      followed out to 2, 6, 10, and 14 days. Six transplants were performed in each 
      group. Serum and splenic samples were used to evaluate the cytokine response by 
      ELISA. Explanted heart tissue was processed for evidence of histologic rejection, 
      and RT-PCR was performed to evaluate the IL-12, IL-18, and IFN-gamma signal 
      qualitatively. Analysis of variance (ANOVA), Fisher's projected least significant 
      difference (PLSD) was used for statistical analysis. Transplant rejection 
      occurred in the allogeneic group histologically by day 6 and clinically by day 
      10. Serum IFN-gamma levels rose significantly by day 6 in the allogeneic group 
      and then continued to rise in the splenocyte cultures. Serum IL-18 also rose 
      significantly in the allogeneic group at day 6 compared with syngeneic group. 
      RT-PCR revealed that the allogeneic tissue contained an increased signal for 
      IL-12, IL-18, and IFN-gamma beginning at day 6 and peaking at day 10 after 
      transplant. Beginning 6 days after transplantation, IL-12 and IL-18 appear to 
      play a significant role in the induction of IFN-gamma in allogeneic heart 
      transplants.
FAU - Affleck, D G
AU  - Affleck DG
AD  - Department of Surgery, University of Utah Health Sciences Center, School of 
      Medicine 3C127, Salt Lake City, UT 84132, USA. david.bull@hsc.utah.edu
FAU - Bull, D A
AU  - Bull DA
FAU - Albanil, A
AU  - Albanil A
FAU - Shao, Y
AU  - Shao Y
FAU - Brady, J
AU  - Brady J
FAU - Karwande, S V
AU  - Karwande SV
FAU - Eichwald, E J
AU  - Eichwald EJ
FAU - Shelby, J
AU  - Shelby J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (CD3 Complex)
RN  - 0 (Interleukin-18)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - CD3 Complex/analysis
MH  - Graft Rejection/*immunology/*pathology/physiopathology
MH  - Heart Transplantation/*immunology/*pathology/statistics & numerical data
MH  - Interferon-gamma/*biosynthesis/blood/genetics
MH  - Interleukin-12/blood/genetics
MH  - Interleukin-18/*biosynthesis/blood/genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C3H
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spleen/cytology/immunology/metabolism
MH  - Transplantation, Homologous
MH  - Transplantation, Isogeneic
EDAT- 2001/02/15 11:00
MHDA- 2001/05/01 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/05/01 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1089/107999001459105 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2001 Jan;21(1):1-9. doi: 10.1089/107999001459105.