PMID- 11177598
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20041117
IS  - 1525-8165 (Print)
IS  - 1525-8165 (Linking)
VI  - 9
IP  - 6
DP  - 2000 Dec
TI  - Chromosomal aberrations characteristic for sAML/sMDS are not detectable by random 
      screening using FISH in peripheral blood-derived grafts used for autologous 
      transplantation.
PG  - 861-5
AB  - Acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS) is observed in 5 
      to 10% of patients treated with high-dose chemotherapies followed by autologous 
      stem cell and bone marrow transplantation. Both diseases are frequently 
      associated with monosomy 7 (-7), trisomy 8 (+8), loss of the long arm of 
      chromosome 5 (-5q), and deletions including the TP53-gene region according to 
      del(17)(p13). In this study, we examined whether these chromosomal aberrations 
      are already detectable in blood stem cells from patients who have all been 
      treated with standard chemotherapies prior to peripheral blood stem cell 
      transplantation (PBSCT). Therefore, we screened peripheral blood derived stem 
      cells obtained at the time of stem cell harvest for the presence of -7, +8, -5q, 
      and del(17)(p13) by fluorescence in situ hybridization (FISH). Our series 
      included 40 patients: 4 patients with Hodgkin's disease, 6 patients with 
      non-Hodgkin-lymphoma (NHL), 1 patient with ALL, 4 patients with plasmocytoma, and 
      25 patients with solid tumors. Peripheral blood mononuclear cells (PBMC) from 
      eight healthy blood donors served as controls. Assuming a hybridization 
      efficiency of >98%, the cut-off level of non diploid cells was determined for 
      each DNA-probe. None of the stem cell preparations exhibited chromosomal damage. 
      Our findings indicate that chromosomal damage is a rare event in stem cell 
      autografts.
FAU - Weber, M H
AU  - Weber MH
AD  - Department of Medicine III (Haematology, Oncology, and Transfusion Medicine), 
      Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 
      30, D-12200 Berlin, Germany.
FAU - Wenzel, U
AU  - Wenzel U
FAU - Thiel, E
AU  - Thiel E
FAU - Knauf, W U
AU  - Knauf WU
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Hematother Stem Cell Res
JT  - Journal of hematotherapy & stem cell research
JID - 100892915
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Case-Control Studies
MH  - Chromosome Aberrations/*diagnosis/genetics
MH  - Chromosome Disorders
MH  - Cohort Studies
MH  - Female
MH  - Hematopoietic Stem Cell Transplantation/methods/*standards
MH  - Hematopoietic Stem Cells/*metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukapheresis
MH  - Leukemia, Myeloid/blood/*genetics/therapy
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/blood/*genetics/therapy
MH  - Neoplasms/blood/genetics/therapy
MH  - Transplantation, Autologous/methods/standards
EDAT- 2001/02/15 11:00
MHDA- 2001/06/23 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/06/23 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1089/152581600750062291 [doi]
PST - ppublish
SO  - J Hematother Stem Cell Res. 2000 Dec;9(6):861-5. doi: 10.1089/152581600750062291.