PMID- 11177602
OWN - NLM
STAT- MEDLINE
DCOM- 20010614
LR  - 20041117
IS  - 1525-8165 (Print)
IS  - 1525-8165 (Linking)
VI  - 9
IP  - 6
DP  - 2000 Dec
TI  - Correlation of CD34+ cell counts with volume of bone marrow collected for 
      allogeneic bone marrow harvests.
PG  - 891-4
AB  - The quantity of bone marrow collected for allogeneic bone marrow transplantation 
      is based on collecting 10 to 15 cc of bone marrow/kg of recipient weight. We 
      hypothesized that the percentage of CD34+ cells collected during a bone marrow 
      harvest decreased at the end of the harvest because of increasing amounts of 
      peripheral blood contamination. We performed a prospective, blinded study in 
      which we measured CD34+ percentages and cell counts at 200-cc intervals during 
      bone marrow harvests from 11 consecutive human leukocyte antigen (HLA)-matched 
      sibling bone marrow donors. We observed that the percentage of CD34+ cells in 
      aspirated bone marrow did not vary significantly from the start to the end of the 
      bone marrow harvest, and the total number of CD34+ cells/kg increased in a linear 
      fashion, thus disproving our original hypothesis. In conclusion, the percentage 
      of CD34+ cells in aspirated bone marrow will remain constant throughout a bone 
      marrow harvest.
FAU - Shaughnessy, P J
AU  - Shaughnessy PJ
AD  - Wilford Hall Medical Center, Hematology and Oncology/MMIH, Fisher Bone Marrow 
      Transplant Unit, 2200 Bergquist Ste.1, Lackland AFB, TX 78236-5300, USA. 
      pshaughnessy@netscape.net
FAU - Kissack, B
AU  - Kissack B
FAU - Bickford, D J
AU  - Bickford DJ
FAU - Ririe, D W
AU  - Ririe DW
FAU - Ornstein, D L
AU  - Ornstein DL
FAU - Lopez, J
AU  - Lopez J
FAU - Hensley, R E
AU  - Hensley RE
FAU - Smith, R
AU  - Smith R
FAU - Schroeder, M T
AU  - Schroeder MT
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Hematother Stem Cell Res
JT  - Journal of hematotherapy & stem cell research
JID - 100892915
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, CD34/*analysis
MH  - Blood Component Removal/methods/standards
MH  - Bone Marrow
MH  - Bone Marrow Transplantation/methods/*standards
MH  - Cell Count
MH  - Female
MH  - Hematopoietic Stem Cells/*cytology/immunology
MH  - Histocompatibility
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nuclear Family
MH  - Prospective Studies
MH  - Single-Blind Method
MH  - Transplantation, Homologous/methods/standards
EDAT- 2001/02/15 11:00
MHDA- 2001/06/23 10:01
CRDT- 2001/02/15 11:00
PHST- 2001/02/15 11:00 [pubmed]
PHST- 2001/06/23 10:01 [medline]
PHST- 2001/02/15 11:00 [entrez]
AID - 10.1089/152581600750062336 [doi]
PST - ppublish
SO  - J Hematother Stem Cell Res. 2000 Dec;9(6):891-4. doi: 10.1089/152581600750062336.