PMID- 11178980
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20101118
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 281
IP  - 1
DP  - 2001 Feb 16
TI  - Effect of interleukin-10 on the gene expression of type I collagen, fibronectin, 
      and decorin in human skin fibroblasts: differential regulation by transforming 
      growth factor-beta and monocyte chemoattractant protein-1.
PG  - 200-5
AB  - Interleukin-10 (IL-10) is a cytokine with many regulatory functions. In 
      particular, IL-10 exerts neutralizing effect on other cytokines, and therefore 
      IL-10 is thought to have important therapeutic implications. Recent reports 
      suggest that IL-10 regulates not only immunocytes but also collagen and 
      collagenase gene expression in fibroblasts. In this study, we investigated the 
      effect of IL-10 on gene expression of extracellular matrix (ECM) proteins, such 
      as type I collagen, fibronectin, and decorin, in human skin fibroblasts. Results 
      of Northern blot analysis showed that both collagen I and fibronectin mRNAs were 
      downregulated, while decorin gene expression was enhanced by IL-10 (10 ng/ml) 
      time-dependently (6-24 h). alpha1(I) collagen and fibronectin mRNAs were 
      decreased to one-third and one-fourth, respectively, by 50 ng/ml IL-10, whereas 
      decorin mRNA was increased up to 2.7-fold by 50 ng/ml IL-10. Response to IL-10 by 
      scleroderma fibroblasts was similar to that in normal dermal fibroblasts, with 
      decreased expression levels of collagen and fibronectin and induced decorin mRNA 
      levels. Transforming growth factor-beta (TGF-beta) is a crucial fibrogenic 
      cytokine which upregulates the mRNA expression of collagen and fibronectin, 
      whereas it downregulates decorin mRNA expression in fibroblasts. Monocyte 
      chemoattractant protein-1 (MCP-1) has recently been shown to upregulate the type 
      I collagen mRNA expression in cultured fibroblasts. We therefore examined whether 
      IL-10 alters gene expression of ECM elicited by TGF-beta and MCP-1. Our results 
      demonstrated that IL-10 downregulated the TGF-beta-elicited increase of mRNA 
      expression of type I collagen and fibronectin, while partially recovering 
      TGF-beta-elicited decrease of decorin expression in normal skin fibroblasts. By 
      contrast, IL-10 did not alter the MCP-1-elicited upregulation of mRNA expression 
      of either alpha1(I) collagen and decorin. Our data indicate that IL-10 
      differentially regulates TGF-beta and MCP-1 in the modulation of ECM proteins and 
      therefore suggest that IL-10 plays a role in the regulation of tissue remodeling.
FAU - Yamamoto, T
AU  - Yamamoto T
AD  - Department of Dermatology, University of Cologne, Joseph-Stelzmann Strasse 9, 
      Cologne, 50924, Germany. yamamoto.derm@med.tmd.ac.jp
FAU - Eckes, B
AU  - Eckes B
FAU - Krieg, T
AU  - Krieg T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chemokine CCL2)
RN  - 0 (DCN protein, human)
RN  - 0 (Decorin)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Fibronectins)
RN  - 0 (Proteoglycans)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Collagen/*biosynthesis
MH  - Decorin
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation
MH  - Extracellular Matrix/metabolism
MH  - Extracellular Matrix Proteins
MH  - Fibroblasts/*metabolism
MH  - Fibronectins/*biosynthesis
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Interleukin-10/*pharmacology
MH  - Proteoglycans/*biosynthesis
MH  - RNA, Messenger/metabolism
MH  - Scleroderma, Localized/metabolism
MH  - Skin/*metabolism
MH  - Time Factors
MH  - Transforming Growth Factor beta/*metabolism
MH  - Up-Regulation
EDAT- 2001/02/17 11:00
MHDA- 2001/04/06 10:01
CRDT- 2001/02/17 11:00
PHST- 2001/02/17 11:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/02/17 11:00 [entrez]
AID - S0006-291X(01)94321-9 [pii]
AID - 10.1006/bbrc.2001.4321 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Feb 16;281(1):200-5. doi: 
      10.1006/bbrc.2001.4321.