PMID- 11178984
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 281
IP  - 1
DP  - 2001 Feb 16
TI  - Differentially expressed cDNAs in PLCbeta3-induced tumor suppression in a human 
      endocrine pancreatic tumor cell line: activation of the human mismatch repair 
      protein 3 gene.
PG  - 227-31
AB  - Phospholipase Cbeta3 (PLCB3) is located to chromosome 11q13 in the vicinity of 
      the multiple endocrine neoplasia type1 (MEN1) gene and shows loss of expression 
      in some neuroendocrine tumors. Transfection of PLCB3 to neuroendocrine cell lines 
      induces growth suppression and phenotypic alterations, but the mechanisms remain 
      unclear. To investigate the underlying events behind this tumor suppression, we 
      performed an RT-Differential cDNA Display of total RNA from BON-1 (human 
      endocrine pancreatic tumor cell line) transfected with PLCB3 and compared to wild 
      type and BON-1 transfected with vector without insert. PLCB3 transfection 
      resulted in increased expression of 4 genes and decreased of 2. The two inhibited 
      were homologous to S100A3 and Chromogranin A. One of the four activated cDNAs 
      could be identified as human mismatch repair protein 3 mRNA (hMSH3), and another 
      was homologous to TIS/MA-3 mRNA (mouse topoisomerase suppressor inhibited 
      gene/mouse apoptosis gene-3). Differential expression of these genes may 
      contribute to the PLCB3-induced tumor suppression of neuroendocrine tumor cell 
      lines.
FAU - Stalberg, P
AU  - Stalberg P
AD  - Endocrine Oncology Unit, Department of Medical Sciences, University Hospital, 
      Uppsala, 751 85, Sweden. Peter.Stalberg@medsci.uu.se
FAU - Lopez-Egido, J R
AU  - Lopez-Egido JR
FAU - Wang, S
AU  - Wang S
FAU - Gobl, A
AU  - Gobl A
FAU - Oberg, K
AU  - Oberg K
FAU - Skogseid, B
AU  - Skogseid B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Chromogranin A)
RN  - 0 (Chromogranins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (MSH3 protein, human)
RN  - 0 (Multidrug Resistance-Associated Proteins)
RN  - 0 (MutS Homolog 3 Protein)
RN  - 0 (PDCD4 protein, human)
RN  - 0 (Pdcd4 protein, mouse)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (S100 Proteins)
RN  - 0 (S100A3 protein, human)
RN  - 0 (S100a3 protein, mouse)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.11 (PLCB3 protein, human)
RN  - EC 3.1.4.11 (Phospholipase C beta)
RN  - EC 3.1.4.11 (Plcb3 protein, mouse)
RN  - Y49M64GZ4Q (multidrug resistance-associated protein 1)
SB  - IM
MH  - Animals
MH  - Apoptosis Regulatory Proteins
MH  - Base Pair Mismatch
MH  - Blotting, Northern
MH  - Calcium-Binding Proteins/metabolism
MH  - Chromogranin A
MH  - Chromogranins/metabolism
MH  - DNA Repair
MH  - DNA, Complementary/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Down-Regulation
MH  - Enzyme Activation
MH  - Gene Expression Profiling
MH  - Humans
MH  - Isoenzymes/*metabolism
MH  - Mice
MH  - *Multidrug Resistance-Associated Proteins
MH  - MutS Homolog 3 Protein
MH  - Phenotype
MH  - Phospholipase C beta
MH  - Plasmids/metabolism
MH  - Polymerase Chain Reaction
MH  - Protein Isoforms
MH  - Proteins/metabolism
MH  - *RNA-Binding Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *S100 Proteins
MH  - Signal Transduction
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Type C Phospholipases/*metabolism
EDAT- 2001/02/17 11:00
MHDA- 2001/04/06 10:01
CRDT- 2001/02/17 11:00
PHST- 2001/02/17 11:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/02/17 11:00 [entrez]
AID - S0006-291X(01)94329-3 [pii]
AID - 10.1006/bbrc.2001.4329 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Feb 16;281(1):227-31. doi: 
      10.1006/bbrc.2001.4329.