PMID- 11179377
OWN - NLM
STAT- MEDLINE
DCOM- 20010524
LR  - 20240407
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 69
IP  - 3
DP  - 2001 Mar
TI  - Legionella pneumophila suppresses interleukin-12 production by macrophages.
PG  - 1929-33
AB  - In vitro infection of macrophages with Legionella pneumophila induced 
      interleukin-1alpha (IL-1alpha), IL-10, monocyte chemotactic protein 1 (MCP-1), 
      and MCP-3 but not IL-12. The lipopolysaccharide (LPS)-induced production of IL-12 
      was down-regulated by infection with virulent L. pneumophila, but other cytokines 
      were not affected. In contrast, avirulent L. pneumophila or UV-killed, virulent 
      L. pneumophila did not induce any suppression of IL-12. The IL-12 suppression 
      occurred at the level of mRNA accumulation for IL-12 genes in response to LPS 
      stimulation, but the infection induced a marked accumulation of mRNA for both 
      MCP-1 and MCP-3, which are known to suppress IL-12 production in LPS-stimulated 
      macrophages. However, pretreatment of macrophages with MCP-1 did not suppress 
      LPS-induced IL-12 production at the concentrations induced by L. pneumophila 
      infection. These results suggest that L. pneumophila selectively suppresses IL-12 
      production induced by LPS from macrophages in vitro by an MCP-independent 
      mechanism.
FAU - Matsunaga, K
AU  - Matsunaga K
AD  - Department of Medical Microbiology and Immunology, University of South Florida 
      College of Medicine, Tampa, Florida 33612, USA.
FAU - Klein, T W
AU  - Klein TW
FAU - Newton, C
AU  - Newton C
FAU - Friedman, H
AU  - Friedman H
FAU - Yamamoto, Y
AU  - Yamamoto Y
LA  - eng
GR  - R01 AI045169/AI/NIAID NIH HHS/United States
GR  - AI45169/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/metabolism
MH  - Cytokines/genetics/metabolism
MH  - Female
MH  - Interleukin-12/*metabolism
MH  - Legionella pneumophila/*immunology/pathogenicity
MH  - Lipopolysaccharides/immunology
MH  - Macrophages, Peritoneal/*immunology
MH  - Mice
MH  - RNA, Messenger/biosynthesis
PMC - PMC98106
EDAT- 2001/02/17 11:00
MHDA- 2001/05/26 10:01
PMCR- 2001/03/01
CRDT- 2001/02/17 11:00
PHST- 2001/02/17 11:00 [pubmed]
PHST- 2001/05/26 10:01 [medline]
PHST- 2001/02/17 11:00 [entrez]
PHST- 2001/03/01 00:00 [pmc-release]
AID - 1307 [pii]
AID - 10.1128/IAI.69.3.1929-1933.2001 [doi]
PST - ppublish
SO  - Infect Immun. 2001 Mar;69(3):1929-33. doi: 10.1128/IAI.69.3.1929-1933.2001.