PMID- 11179694
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 262
IP  - 1-2
DP  - 2001 Jan 10
TI  - Gene structure, chromosomal localization and immunolocalization of chicken 
      centromere proteins CENP-C and ZW10.
PG  - 283-90
AB  - We determined the genomic structures and complete sequences of the coding regions 
      of the chicken CENP-C and ZW10 genes. These two genes encode proteins that are 
      thought to be involved in maintaining the fidelity of chromosome segregation. The 
      chicken CENP-C gene is 30 kb in length and contains 19 exons. The chicken ZW10 
      gene spans 10 kb and contains 15 exons. The 5'-untranslated regions of these 
      genes contain several binding sites for transcription factors such as Sp-1, E2F, 
      p300, and members of the GATA family. By fluorescence in situ hybridization 
      (FISH) analysis, the CENP-C was mapped to chromosome 4 and the ZW10 gene was 
      mapped to a microchromosome. Antibodies against the chicken ZW10 protein revealed 
      a cell cycle-dependent staining pattern in DT40 cells. ZW10 protein was 
      distributed throughout the cytoplasm of DT40 cells during interphase. In most 
      metaphase cells, ZW10 proteins appeared equally divided between the centromere 
      and the spindle apparatus. During anaphase, chicken ZW10 proteins were no longer 
      localized near chromosomes or the mitotic apparatus but were present diffusely in 
      the cytoplasm.
FAU - Okamura, A
AU  - Okamura A
AD  - Department of Evolutionary Genetics, National Institute of Genetics and The 
      Graduate University for Advanced Studies, Mishima, 411-8540, Shizuoka-ken, Japan.
FAU - Pendon, C
AU  - Pendon C
FAU - Valdivia, M M
AU  - Valdivia MM
FAU - Ikemura, T
AU  - Ikemura T
FAU - Fukagawa, T
AU  - Fukagawa T
LA  - eng
SI  - GENBANK/AB042323
SI  - GENBANK/AB042324
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Avian Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (ZW10 protein, chicken)
RN  - 0 (centromere protein C)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Avian Proteins
MH  - Base Sequence
MH  - Cell Cycle/genetics
MH  - Cell Cycle Proteins/*genetics/metabolism
MH  - Cells, Cultured
MH  - Chickens/*genetics
MH  - Chromosomal Proteins, Non-Histone/*genetics/metabolism
MH  - *Chromosome Mapping
MH  - Exons
MH  - Immunohistochemistry/methods
MH  - Molecular Sequence Data
EDAT- 2001/02/17 11:00
MHDA- 2001/07/06 10:01
CRDT- 2001/02/17 11:00
PHST- 2001/02/17 11:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/02/17 11:00 [entrez]
AID - S0378111900005175 [pii]
AID - 10.1016/s0378-1119(00)00517-5 [doi]
PST - ppublish
SO  - Gene. 2001 Jan 10;262(1-2):283-90. doi: 10.1016/s0378-1119(00)00517-5.