PMID- 11181048
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 281
IP  - 2
DP  - 2001 Feb 23
TI  - Occurrence of D-Amino Acids and a pyridoxal 5'-phosphate-dependent aspartate 
      racemase in the acidothermophilic archaeon, Thermoplasma acidophilum.
PG  - 317-21
AB  - Free D-amino acid content in some archaea was investigated and D-forms of several 
      amino acids were found in them. In the acidothermophilic archaeon, Thermoplasma 
      acidophilum, the proportion of D-aspartate (D-Asp) to total Asp was as high as 
      39.7%. Crude extracts of Thermoplasma acidophilum had Asp-specific racemase 
      activity that was pyridoxal 5'-phosphate (PLP)-dependent. The relative 
      insensitivity to a SH-modifying reagent distinguished this activity from those of 
      the PLP-independent Asp racemases found in other hyperthermophilic archaea 
      (Matsumoto, M., et al., J. Bacteriol. 181, 6560-6563 1999). Thus, high levels of 
      d-Asp should be produced by a new type(s) of Asp-specific racemase in 
      Thermoplasma acidophilum, although the function of d-Asp in this archaeon remains 
      unknown.
CI  - Copyright 2001 Academic Press.
FAU - Long, Z
AU  - Long Z
AD  - School of Pharmaceutical Sciences, Kitasato University, Minato-ku, Tokyo 
      108-8641, Japan.
FAU - Lee, J A
AU  - Lee JA
FAU - Okamoto, T
AU  - Okamoto T
FAU - Sekine, M
AU  - Sekine M
FAU - Nimura, N
AU  - Nimura N
FAU - Imai, K
AU  - Imai K
FAU - Yohda, M
AU  - Yohda M
FAU - Maruyama, T
AU  - Maruyama T
FAU - Sumi, M
AU  - Sumi M
FAU - Kamo, N
AU  - Kamo N
FAU - Yamagishi, A
AU  - Yamagishi A
FAU - Oshima, T
AU  - Oshima T
FAU - Homma, H
AU  - Homma H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Amino Acids)
RN  - 0 (Enzyme Inhibitors)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - 5V5IOJ8338 (Pyridoxal Phosphate)
RN  - EC 5.1.1.- (Amino Acid Isomerases)
RN  - EC 5.1.1.13 (aspartate racemase)
RN  - GMW67QNF9C (Leucine)
RN  - K3Z4F929H6 (Lysine)
RN  - O3C74ACM9V (Ethylmaleimide)
RN  - OF5P57N2ZX (Alanine)
SB  - IM
MH  - Alanine/chemistry/metabolism
MH  - Amino Acid Isomerases/antagonists & inhibitors/*metabolism
MH  - Amino Acids/chemistry/*metabolism
MH  - Aspartic Acid/chemistry/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Ethylmaleimide/pharmacology
MH  - Glutamic Acid/chemistry/metabolism
MH  - Leucine/chemistry/metabolism
MH  - Lysine/chemistry/metabolism
MH  - Phenylalanine/chemistry/metabolism
MH  - Pyridoxal Phosphate/physiology
MH  - Stereoisomerism
MH  - Thermoplasma/enzymology/*metabolism
EDAT- 2001/02/22 11:00
MHDA- 2001/04/03 10:01
CRDT- 2001/02/22 11:00
PHST- 2001/02/22 11:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/02/22 11:00 [entrez]
AID - S0006-291X(01)94353-0 [pii]
AID - 10.1006/bbrc.2001.4353 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Feb 23;281(2):317-21. doi: 
      10.1006/bbrc.2001.4353.