PMID- 11186234
OWN - NLM
STAT- MEDLINE
DCOM- 20010208
LR  - 20061115
IS  - 0897-7151 (Print)
IS  - 0897-7151 (Linking)
VI  - 17
IP  - 12
DP  - 2000 Dec
TI  - Effect of brain-derived neurotrophic factor, nerve growth factor, and 
      neurotrophin-3 on functional recovery and regeneration after spinal cord injury 
      in adult rats.
PG  - 1219-31
AB  - This study examined whether continuous intramedullary infusion of brain-derived 
      neurotrophic factor (BDNF), nerve growth factor (NGF), or neurotrophin-3 (NT-3) 
      had either an early neuroprotective effect or a delayed effect on regeneration 
      after spinal cord injury (SCI) in adult rats. BDNF, NGF, NT-3 or vehicle was 
      infused at a rate of 625 ng/h into the SCI site at T3 through an implanted 
      cannula attached to an osmotic pump. This infusion was maintained for 14 days 
      after a 35-g clip compression injury. At 4 weeks after injury, the axonal tracer 
      fluorogold (FG) was introduced into the spinal cord caudal to the lesion and the 
      animals sacrificed 3 days later following behavioral assessment. The inclined 
      plane score was significantly higher in BDNF-treated animals (45 +/- 3 degrees) 
      compared to control animals (36 -/+ 1 degrees) at 1 week after injury (p < 0.05), 
      although the scores were not significantly different at later times. BDNF-treated 
      animals also showed more FG-labeled cells in the red nucleus and sensorimotor 
      cortex (1,638 +/- 350 and 124 +/- 83, respectively) compared to controls (1,228 
      +/- 217 and 36 +/- 15, respectively) and a lower percent cavitation at the injury 
      site (21.4 +/- 10.4%) compared to control animals (32.3 +/- 11.7%). Invasion & 
      proliferation of Schwann cells and formation of peripheral myelin were more 
      prominent at the injury site in the BDNF-treated animals than in the other 
      groups. These results indicate that continuous intramedullary infusion of BDNF 
      provides neuroprotection and enhances some regenerative activity after SCI.
FAU - Namiki, J
AU  - Namiki J
AD  - University of Toronto and Toronto Western Research Institute, Ontario, Canada.
FAU - Kojima, A
AU  - Kojima A
FAU - Tator, C H
AU  - Tator CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
RN  - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Stilbamidines)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Axons/pathology
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Female
MH  - Fluorescent Dyes
MH  - Motor Activity/drug effects
MH  - Nerve Growth Factor/*pharmacology
MH  - Nerve Regeneration/*drug effects
MH  - Neuroprotective Agents/*pharmacology
MH  - Neurotrophin 3/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Red Nucleus/pathology
MH  - Somatosensory Cortex/pathology
MH  - Spinal Cord/pathology
MH  - Spinal Cord Injuries/pathology/*physiopathology
MH  - *Stilbamidines
EDAT- 2001/02/24 12:00
MHDA- 2001/03/03 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - 10.1089/neu.2000.17.1219 [doi]
PST - ppublish
SO  - J Neurotrauma. 2000 Dec;17(12):1219-31. doi: 10.1089/neu.2000.17.1219.