PMID- 11188932
OWN - NLM
STAT- MEDLINE
DCOM- 20010201
LR  - 20041117
IS  - 0025-7680 (Print)
IS  - 0025-7680 (Linking)
VI  - 60 Suppl 2
DP  - 2000
TI  - Cancer immunotherapy using dendritic cell-derived exosomes.
PG  - 51-4
AB  - Dendritic cells (DCs) are the most potent antigen presenting cells and the only 
      ones capable of inducing primary cytotoxic immune responses. We found that DCs 
      secrete a population of membrane vesicles, called exosomes. Exosomes are 60-80 nm 
      vesicles of endocytic origin. The protein composition of exosomes was subjected 
      to a systematic proteomic analysis. Besides MHC and co-stimulatory molecules, 
      exosomes bear several adhesion proteins, most likely involved in their specific 
      subjected to targeting. We also found that exosomes accumulate several cytosolic 
      factors, probably involved in their endosomal biogenesis. Like DCs, exosomes 
      induced immune responses in vivo. Indeed, a single injection of DC-derived 
      exosomes sensitized with tumor peptides induced potent anti tumor immune 
      responses in mice and the eradication of established tumors. Tumor-specific 
      cytotoxic T lymphocytes were found in the spleen of exosome-treated mice, and the 
      anti tumor effect of exosomes was sensitive to in vivo depletion of CD8+ T cells. 
      These results show that exosomes induce potent anti tumor effects in vivo, and 
      strongly support the implementation of human DC-derived exosomes for cancer 
      immunotherapy.
FAU - Amigorena, S
AU  - Amigorena S
AD  - Unite INSERM U520, Institut Curie, Paris, France. sebastian.amigorena@curie.fr
LA  - eng
PT  - Journal Article
PL  - Argentina
TA  - Medicina (B Aires)
JT  - Medicina
JID - 0204271
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cytoplasmic Vesicles/immunology/*physiology
MH  - Dendritic Cells/*physiology/ultrastructure
MH  - Endosomes/physiology
MH  - Humans
MH  - Immunotherapy/*methods
MH  - Mice
MH  - Neoplasms/*therapy
EDAT- 2001/02/24 12:00
MHDA- 2001/02/28 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PST - ppublish
SO  - Medicina (B Aires). 2000;60 Suppl 2:51-4.