PMID- 11191075
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20061115
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 22
IP  - 1
DP  - 2001 Jan-Feb
TI  - Lack of significant morphological differences between human X and Y spermatozoa 
      and their precursor cells (spermatids) exposed to different prehybridization 
      treatments.
PG  - 119-23
AB  - Human X and Y spermatozoa were previously compared by several nonmolecular 
      techniques. Recent studies show that in many of the previous investigations, the 
      methods used to identify the spermatozoa were nonspecific and thus produced 
      contradictory findings. In the present study, the comparison of the 2 germ cell 
      types, X and Y, were performed following fluorescence in situ hybridization 
      (FISH), which is the most reliable genotyping technique currently available. The 
      FISH technique was performed under 3 different treatments: permeabilization with 
      liquid N2, fixation with Carnoy's, and chromatin decondensation with lithium 
      di-iodosalicylate. Mature and immature germ cells (spermatozoa and spermatids) 
      were compared. Lithium showed higher hybridization efficiency, while liquid N2 
      and Carnoy's fixative maintained better morphological integrity of cells with 
      lower hybridization. The sperm exhibiting hybridization signals were not 
      different in any of the morphometric or qualitative comparisons from those that 
      did not exhibit signals. No significant deviation of the sex ratio from 1:1 was 
      seen in either the mature or immature germ cell population. The spatial 
      distribution of X and Y chromosome-specific signals in the sperm head were 
      identical. The hybridization treatments did not have any preferential effect on 
      the cells of specific genotype (X or Y). Neither head parameters (length, HL; 
      width, HW; area, HA) nor tail length (TL) significantly differed between X and Y 
      populations of spermatozoa under any of the treatments. Similarly, the haploid, 
      X-specific round cells did not differ from Y-specific ones by their size 
      (diameter) and shape. These results indicate that neither mature sperm nor their 
      precursors possess significant morphological differences between X and Y 
      genotypes.
FAU - Hossain, A M
AU  - Hossain AM
AD  - Department of Obstetrics and Gynecology, University of Illinois at Chicago, 
      60612, USA. amjad@uic.edu
FAU - Barik, S
AU  - Barik S
FAU - Kulkarni, P M
AU  - Kulkarni PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
SB  - IM
MH  - Cell Separation
MH  - Cell Size
MH  - Genotype
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nucleic Acid Hybridization
MH  - Sperm Head
MH  - Spermatids/*cytology
MH  - *X Chromosome
MH  - *Y Chromosome
EDAT- 2001/02/24 12:00
MHDA- 2001/03/10 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PST - ppublish
SO  - J Androl. 2001 Jan-Feb;22(1):119-23.