PMID- 11191087
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20131121
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 22
IP  - 1
DP  - 2001 Jan-Feb
TI  - Comparative study of cytochemical tests for sperm chromatin integrity.
PG  - 45-53
AB  - Tests were carried out on sperm from 40 fertile and infertile men to evaluate 2 
      DNA in situ denaturation methods using acridine orange (AO; the modified 
      Rigler-Roschlau method and the Tejada method), alongside routine aniline blue 
      (AB) and toluidine blue (TB) tests in our modification, and in order to estimate 
      and compare the practical value of different in situ cytochemical tests for sperm 
      chromatin structure. In addition, the methods were applied to rat and boar 
      spermiogenesis models. The sperm heads with abnormal versus normal chromatin 
      structure were specified as orange-red versus green by the AO method, blue versus 
      uncolored by the AB method, and purple-violet versus light blue by the TB method. 
      A good correlation for the proportion of sperm heads with abnormal chromatin 
      structure was found among all the methods (r = .63-.70; P < .01), which 
      characterized all 4 techniques as sensitive enough to estimate in situ sperm DNA 
      integrity. In our study, the average value of abnormal cells was 17% +/- 3.8% and 
      30.2% +/- 6.8% for the fertile and infertile groups of men, respectively, setting 
      a threshold of 95% probability at 23% as judged by the Rigler-Roschlau method. 
      This compared with 23.9% +/- 7.5% and 52.1% +/- 20.8% (P < or = .05) for the 
      fertile and infertile groups, respectively, setting a threshold at 31%, as judged 
      by the Tejada method. The technical advantages and disadvantages of each method 
      are briefly reported. Key words: Fertility, DNA normality, sperm maturation.
FAU - Erenpreiss, J
AU  - Erenpreiss J
AD  - Family and Sexual Health Center, Riga, Latvia. cancer@latnet.lv
FAU - Bars, J
AU  - Bars J
FAU - Lipatnikova, V
AU  - Lipatnikova V
FAU - Erenpreisa, J
AU  - Erenpreisa J
FAU - Zalkalns, J
AU  - Zalkalns J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - 0 (Aniline Compounds)
RN  - 0 (Chromatin)
RN  - 0 (Coloring Agents)
RN  - 0 (Fluorescent Dyes)
RN  - 15XUH0X66N (Tolonium Chloride)
RN  - 8004-91-9 (aniline blue)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - *Aniline Compounds
MH  - Animals
MH  - Chromatin/*chemistry
MH  - Coloring Agents
MH  - DNA/*analysis
MH  - Fluorescent Dyes
MH  - Humans
MH  - Infertility, Male/pathology
MH  - Male
MH  - Nucleic Acid Denaturation
MH  - Rats
MH  - Spermatogenesis
MH  - Spermatozoa/*chemistry/*cytology
MH  - Staining and Labeling
MH  - Swine
MH  - Tolonium Chloride
EDAT- 2001/02/24 12:00
MHDA- 2001/03/10 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PST - ppublish
SO  - J Androl. 2001 Jan-Feb;22(1):45-53.