PMID- 11194473
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20190718
IS  - 0005-0423 (Print)
IS  - 0005-0423 (Linking)
VI  - 78
IP  - 12
DP  - 2000 Dec
TI  - Surveys in Papua New Guinea to detect the presence of Trypanosoma evansi 
      infection.
PG  - 843-5
AB  - OBJECTIVE: To confirm serological evidence that Trypanosoma evansi is present in 
      Papua New Guinea. DESIGN: Three surveys were undertaken in PNG during 1997/1998. 
      Animals were selected for sampling on the basis of convenience. Samples of blood 
      were examined for the presence of T evansi by the haematocrit centrifugation 
      technique (HCT) and mouse inoculation test (MI). Sera were tested in the field 
      using the card agglutination test for trypanosomiasis/T evansi (CATT). Bovine 
      sera were tested at James Cook University using an antibody-detection ELISA 
      (Ab-ELISA). Results from testing bovine sera with the Ab-ELISA and sera from 
      wallabies with the CATT were analysed using FreeCalc to determine the probability 
      that animals in these populations were infected with T evansi. RESULTS: A total 
      of 545 serum samples were collected, during the three surveys of which 39 cattle, 
      two pig and three agile wallaby samples were positive with the CATT. All bovine 
      sera collected were negative when tested with an Ab-ELISA. T evansi was not 
      isolated using the HCT or the MI from any of these animals. CONCLUSION: Based on 
      the Ab-ELISA results it was concluded that T evansi infection was not present in 
      cattle in villages around Balimo at a minimum expected prevalence of 10% (P < 
      0.05) and, based on the CATT results, that infection was not present in wallabies 
      on the Bula plain at a minimum expected prevalence of 10% (P < 0.1). These 
      results indicate that it is unlikely that T evansi is endemic in PNG.
FAU - Reid, S A
AU  - Reid SA
AD  - Australian Institute of Tropical and Veterinary Science, James Cook University, 
      Townsville, Queensland 4811. sreid@central.murdoch.edu.au
FAU - Copeman, D B
AU  - Copeman DB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Aust Vet J
JT  - Australian veterinary journal
JID - 0370616
RN  - 0 (Antibodies, Protozoan)
SB  - IM
MH  - Agglutination Tests/veterinary
MH  - Animal Diseases/*epidemiology
MH  - Animals
MH  - Antibodies, Protozoan/blood
MH  - Cattle
MH  - Deer
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Goats
MH  - Horses
MH  - Macropodidae
MH  - Mice
MH  - New Guinea/epidemiology
MH  - Seroepidemiologic Studies
MH  - Swine
MH  - Trypanosoma/*isolation & purification
MH  - Trypanosomiasis/epidemiology/*veterinary
EDAT- 2001/02/24 12:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - 10.1111/j.1751-0813.2000.tb10506.x [doi]
PST - ppublish
SO  - Aust Vet J. 2000 Dec;78(12):843-5. doi: 10.1111/j.1751-0813.2000.tb10506.x.