PMID- 11195340
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20190915
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 43
IP  - 6
DP  - 2000 Dec
TI  - Molecular characterization and chromosomal distribution of species-specific 
      repetitive DNA sequences from Beta corolliflora, a wild relative of sugar beet.
PG  - 1073-80
AB  - Repetitive DNA sequences have been isolated from a Sau3AI plasmid library of 
      tetraploid Beta corolliflora (2n = 4x = 36), a wild relative of sugar beet (B. 
      vulgaris). The library was screened by differential hybridization with genomic 
      DNA of B. corolliflora and B. vulgaris. When used as probes for Southern 
      hybridization of genomic DNA, six clones were determined to represent highly 
      repetitive DNA families present only in the B. corolliflora genome. Five other 
      sequences were highly repetitive in B. corolliflora and low or single copy in B. 
      vulgaris. The insert size varied between 43 bp and 448 bp. Two sequences pBC1279 
      and pBC1944 displayed strong homology to a previously cloned satellite DNA from 
      B. nana. With one exception, sequences are tandemly arranged as revealed by a 
      typical ladder pattern after genomic Southern hybridization. The chromosomal 
      distribution of five probes was determined by fluorescence in situ hybridization 
      (FISH) of mitotic metaphases from B. corolliflora and a triploid hybrid between 
      B. vulgaris and B. corolliflora. Three sequences were spread along all chromosome 
      arms of B. corolliflora while one sequence was present on only six chromosomes. 
      The chromosome-specific sequence pBC216 was found in close vicinity to the 5S 
      rDNA located on B. corolliflora chromosome IV. This set of species-specific 
      sequences has the potential to be used as probes for the identification of 
      monosomic alien addition lines and for marker-assisted gene transfer from wild 
      beet to cultivated beet.
FAU - Gao, D
AU  - Gao D
AD  - Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of 
      Kiel, Germany.
FAU - Schmidt, T
AU  - Schmidt T
FAU - Jung, C
AU  - Jung C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chenopodiaceae/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes
MH  - DNA, Plant/analysis
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Molecular Sequence Data
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
EDAT- 2001/02/24 12:00
MHDA- 2001/03/10 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - 10.1139/g00-084 [doi]
PST - ppublish
SO  - Genome. 2000 Dec;43(6):1073-80. doi: 10.1139/g00-084.