PMID- 11195341
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 43
IP  - 6
DP  - 2000 Dec
TI  - Physical localization of single-copy sequences on pachytene chromosomes in maize 
      (Zea mays L.) by chromosome in situ suppression hybridization.
PG  - 1081-3
AB  - A new approach for locating single-copy DNA sequences on pachytene chromosomes of 
      maize (Zea mays L.) was developed. A cosmid clone with homologous sequences to a 
      molecular marker (umc105a) linked to a quantitative trait locus (QTL) for 
      resistance against sugarcane borer (SCB) was physically mapped by fluorescence in 
      situ hybridization (FISH) to the short arm of chromosome 9. The marker umc105a 
      was genetically placed in the centromeric region. To suppress signals generated 
      by maize repetitive DNA, competitive in situ suppression (CISS) hybridization was 
      necessary to obtain specific signals from umc105a. A centromere specific DNA 
      probe (CentC) was used in a double-labeling technique as a reference marker. 
      Fluorescence signals generated by umc105a cosmid and CentC were specific and 
      highly reproducible. Thus the single-copy DNA sequence of umc105a was physically 
      localized on the short arm of chromosome 9 near the telomere. This is the first 
      report of physical localization of single-copy DNA sequence by CISS hybridization 
      to a maize pachytene chromosome.
FAU - Sadder, M T
AU  - Sadder MT
AD  - Institute of Plant Breeding, Seed Science and Population Genetics, University of 
      Hohenheim, Stuttgart, Germany.
FAU - Ponelies, N
AU  - Ponelies N
FAU - Born, U
AU  - Born U
FAU - Weber, G
AU  - Weber G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Zea mays/*genetics
EDAT- 2001/02/24 12:00
MHDA- 2001/03/10 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PST - ppublish
SO  - Genome. 2000 Dec;43(6):1081-3.