PMID- 11198085
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20190822
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 55
IP  - 1
DP  - 2001 Jan 1
TI  - Progress on methods of gene detection in preimplantation embryos.
PG  - 23-34
AB  - The advent of the polymerase chain reaction (PCR) and the development of 
      fluorescence in situ hybridization (FISH) have had a tremendous impact on 
      preimplantation genetic diagnosis (PGD). While PCR is a powerful tool in 
      detecting genetic diseases or molecular markers affecting quantitative trait 
      loci, the main use of FISH is screening for chromosomal aberrations. This 
      presentation reviews the recent progress in preimplantation genetic diagnosis 
      with an emphasis on bovine embryos. In particular the importance of biopsy size 
      and strategies to avoid PCR contamination are discussed. Alternative DNA 
      amplification and detection methods as well as methods to meet the challenge of 
      multiple locus detection for marker assisted selection are presented.
FAU - Bredbacka, P
AU  - Bredbacka P
AD  - bredbacka@finnzymes.fi
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
SB  - IM
MH  - Animals
MH  - Biopsy
MH  - Cattle
MH  - Cytogenetic Analysis
MH  - Embryo, Mammalian
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - Preimplantation Diagnosis/methods/*veterinary
MH  - Sensitivity and Specificity
RF  - 63
EDAT- 2001/02/24 12:00
MHDA- 2001/04/03 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - S0093-691X(00)00443-X [pii]
AID - 10.1016/s0093-691x(00)00443-x [doi]
PST - ppublish
SO  - Theriogenology. 2001 Jan 1;55(1):23-34. doi: 10.1016/s0093-691x(00)00443-x.