PMID- 11199165
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20170214
IS  - 0300-9858 (Print)
IS  - 0300-9858 (Linking)
VI  - 38
IP  - 1
DP  - 2001 Jan
TI  - Evidence for the localization of porcine reproductive and respiratory syndrome 
      virus (PRRSV) antigen and RNA in ovarian follicles in gilts.
PG  - 58-66
AB  - The pathogenesis of porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection in ovary was studied in sexually mature, cycling, nonsynchronized gilts 
      infected with the PRRSV 16244B, a virulent field strain. Previous studies have 
      shown that PRRSV can be isolated from ovaries and is transplacentally passed from 
      gilts to the fetuses. The cause of infertility following PRRSV infection is not 
      known. In this study, we identified the tropism of PRRSV in ovarian tissue from 
      experimentally infected gilts in samples collected between 7 and 21 days 
      postinfection (DPI). Tissues were collected and examined by virus isolation, in 
      situ hybridization (ISH), immunohistochemistry (IHC), and double labeling to 
      identify PRRSV-infected cell types. PRRSV was isolated in ovarian follicles at 7 
      days DPI. The IHC and ISH indicated that PRRSV-positive cells in ovaries were 
      predominantly macrophages, which were numerous in atretic follicles. No evidence 
      of infection and/or perpetuation of PRRSV in ova was observed, indicating that 
      the female gonad is an unlikely site of persistence. No alteration of the normal 
      ovarian architecture that would support a possible role of PRRSV infection in 
      porcine female infertility was observed.
FAU - Sur, J H
AU  - Sur JH
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln 
      68583-0905, USA.
FAU - Doster, A R
AU  - Doster AR
FAU - Galeota, J A
AU  - Galeota JA
FAU - Osorio, F A
AU  - Osorio FA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Viral)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antigens, Viral/*metabolism
MH  - Cytopathogenic Effect, Viral
MH  - DNA Probes/chemistry
MH  - DNA, Viral/chemistry
MH  - Female
MH  - Granulosa Cells/virology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Macrophages/immunology/pathology/virology
MH  - Microscopy, Fluorescence
MH  - Ovarian Follicle/immunology/metabolism/pathology/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/chemistry/*genetics/*immunology/pathogenicity
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - RNA, Viral/chemistry/isolation & purification/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Specific Pathogen-Free Organisms
MH  - Swine
EDAT- 2001/02/24 12:00
MHDA- 2001/04/03 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - 10.1354/vp.38-1-58 [doi]
PST - ppublish
SO  - Vet Pathol. 2001 Jan;38(1):58-66. doi: 10.1354/vp.38-1-58.