PMID- 11201084
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20220318
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 12
IP  - 1
DP  - 2001 Jan 22
TI  - Effect of brain-derived neurotrophic factor on the release of substance P from 
      rat spinal cord.
PG  - 21-4
AB  - Brain-derived neurotrophic factor (BDNF) can produce hyperalgesia in the adult 
      rat. Here we assessed whether changes in the spinal release of the nociceptive 
      peptide substance P (SP) contributes to this effect. Antibody-coated microprobes 
      revealed a significant basal release of SP in the dorsal horn in vivo that was 
      increased following acute knee inflammation. Microinjection of BDNF into the grey 
      matter (0.5 microl, 10(-5) M) altered SP release neither in rats with normal 
      knees nor in rats with inflamed knee joints. In the lumbar dorsal horn slice 
      preparation in vitro, superfusion with BDNF (100 ng/ml) could reduce SP release 
      evoked by electrical dorsal root stimulation without modyfing SP basal outflow. 
      It is unlikely, therefore, that enhanced spinal SP release mediates the 
      hyperalgesic effect of BDNF.
FAU - Meyer-Tuve, A
AU  - Meyer-Tuve A
AD  - Institut for Physiologie, Universitat Jena, Germany.
FAU - Malcangio, M
AU  - Malcangio M
FAU - Ebersberger, A
AU  - Ebersberger A
FAU - Mazario, J
AU  - Mazario J
FAU - Schaible, H G
AU  - Schaible HG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 33507-63-0 (Substance P)
SB  - IM
MH  - Animals
MH  - Arthritis/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Electric Stimulation
MH  - Hyperalgesia/chemically induced/*metabolism
MH  - Knee Joint/*metabolism
MH  - Male
MH  - Posterior Horn Cells/drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Spinal Cord/*drug effects/metabolism
MH  - Substance P/*metabolism
MH  - Touch
EDAT- 2001/02/24 12:00
MHDA- 2001/03/27 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - 10.1097/00001756-200101220-00012 [doi]
PST - ppublish
SO  - Neuroreport. 2001 Jan 22;12(1):21-4. doi: 10.1097/00001756-200101220-00012.