PMID- 11204924
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20061115
IS  - 0369-8114 (Print)
IS  - 0369-8114 (Linking)
VI  - 48
IP  - 10
DP  - 2000 Dec
TI  - [Recent developments of spoligotyping as applied to the study of epidemiology, 
      biodiversity and molecular phylogeny of the Mycobacterium tuberculosis complex].
PG  - 921-32
AB  - Spoligotyping (for 'spacer-oligonucleotide-typing'), a rapid method for 
      genotyping of Mycobacterium tuberculosis complex using the principle of reverse 
      hybridization, is based on the structure of the direct repeat (DR) locus. The DR 
      locus is made up of a variable number of 36 bp DR repeats that are separated by 
      unique inter-DR sequences of 35 to 41 bp. Fast and highly discriminatory, 
      spoligotyping is an useful alternative to the IS6110-RFLP reference method for 
      molecular typing of M. tuberculosis, in particular for isolates possessing five 
      or few copies of IS6110. In this paper, we review the state of the art of 
      spoligotyping through its main current applications. After a brief introduction 
      to the principle of the technique and its description, we successively review 
      recently published results concerning the molecular epidemiology of tuberculosis 
      in humans and cattle, and discuss the main genotyping strategies currently in use 
      to fingerprint the M. tuberculosis complex organisms. We also describe the recent 
      applications of spoligotyping to study ancient DNA and report on recent 
      developments of this technique to study the biodiversity of the M. tuberculosis 
      complex, its contribution towards improved taxonomy and phylogenetics of the M. 
      tuberculosis complex. Last but not least, potential applications of spoligotyping 
      to study DNA recombination mechanisms are also discussed.
FAU - Sola, C
AU  - Sola C
AD  - Unite de la tuberculose et des mycobacteries, Institut Pasteur de Guadeloupe, 
      Morne Joliviere, BP 484, 97165 Pointe-a-Pitre, Guadeloupe. csola@pasteur.gp
FAU - Filliol, I
AU  - Filliol I
FAU - Legrand, E
AU  - Legrand E
FAU - Rastogi, N
AU  - Rastogi N
LA  - fre
PT  - English Abstract
PT  - Journal Article
PT  - Review
TT  - Developpements recents du spoligotypage appliques a l'etude de l'epidemiologie, 
      de la biodiversite, et de la phylogenie moleculaire du complexe Mycobacterium 
      tuberculosis.
PL  - France
TA  - Pathol Biol (Paris)
JT  - Pathologie-biologie
JID - 0265365
RN  - 0 (DNA, Intergenic)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - DNA, Intergenic
MH  - *Ecosystem
MH  - *Evolution, Molecular
MH  - Genotype
MH  - Humans
MH  - Mycobacterium tuberculosis/classification/*genetics
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotides
MH  - *Phylogeny
MH  - Repetitive Sequences, Nucleic Acid
MH  - Tuberculosis/*epidemiology/microbiology
RF  - 57
EDAT- 2001/02/24 12:00
MHDA- 2001/03/07 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PST - ppublish
SO  - Pathol Biol (Paris). 2000 Dec;48(10):921-32.