PMID- 11205119
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20200324
IS  - 0304-8608 (Print)
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 145
IP  - 11
DP  - 2000
TI  - Full-length sequence of a Canadian porcine reproductive and respiratory syndrome 
      virus (PRRSV) isolate.
PG  - 2297-323
AB  - Presently, one of the most economically important pathogens affecting swine is 
      the porcine reproductive and respiratory syndrome virus (PRRSV). This virus is 
      prevalent in herds throughout the world and continues to pose a significant 
      threat as newer and more virulent disease phenotypes emerge. In this report we 
      describe the full-length nucleotide sequence of a Canadian PRRSV isolate, 
      designated PA8. A consecutive sequence of 15,411 nucleotides was obtained from a 
      set of overlapping cDNA clones. In order to determine the extent of genetic 
      variation among isolates recovered from swine in Canada and the US, as well as to 
      understand the molecular mechanisms governing the evolution of PRRSV, the 
      full-length sequence of PA8 was compared with that of two US isolates, VR2332 and 
      16244B. The genomic sequence of PA8 shared 98.2% and 99.2% identity with 16244B 
      and VR2332, respectively. The untranslated regions (UTR) at the 5' and 3' ends of 
      the genome were very well conserved. Notable exceptions include an eight 
      nucleotide difference at the 5' end of the 5' UTR of VR2332 relative to PA8 and 
      16244B and a two nucleotide difference in the 3' UTR of PA8 relative to VR2332 
      and 16244B. In contrast to PA8 and VR2332, 16244B possessed two nucleotide 
      differences within the RNA pseudoknot structure of the ribosomal frameshift 
      region between open reading frame (ORF)1a and ORF1b. Amino acid differences were 
      distributed throughout the genome, however they appeared to be most extensive in 
      Nsp1beta and ORF5 of the nonstructural and structural coding regions, 
      respectively, suggesting that the evolutionary pressure to conserve these viral 
      genes is somewhat lower.
FAU - Wootton, S
AU  - Wootton S
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Canada.
FAU - Yoo, D
AU  - Yoo D
FAU - Rogan, D
AU  - Rogan D
LA  - eng
SI  - GENBANK/AF066066
SI  - GENBANK/AF066068
SI  - GENBANK/AF176348
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
RN  - 24937-83-5 (Poly A)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - 5' Untranslated Regions/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - *Genome, Viral
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Poly A/genetics
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*isolation & 
      purification
MH  - RNA, Viral/genetics
MH  - Sequence Analysis, DNA
MH  - Swine
PMC - PMC7086845
EDAT- 2001/02/24 12:00
MHDA- 2001/03/03 10:01
PMCR- 2020/03/23
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 1452297 [pii]
AID - 10.1007/s007050070022 [doi]
PST - ppublish
SO  - Arch Virol. 2000;145(11):2297-323. doi: 10.1007/s007050070022.