PMID- 11205322
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20191104
IS  - 0921-8912 (Print)
IS  - 1878-3651 (Electronic)
IS  - 0921-8912 (Linking)
VI  - 20
IP  - 4
DP  - 2000
TI  - Chromosomal composition of aneuploid clones with different DNA contents in head 
      and neck squamous cell carcinomas as determined by combined flow cytometry and 
      fluorescence in situ hybridization.
PG  - 197-203
AB  - Studies with DNA flow cytometry (FCM) have shown that DNA contents of aneuploid 
      tumour clones vary in a wide range. The aim of this study was to analyse whether 
      homologous chromosomal changes exist despite the individual differences that may 
      be of general relevance for the development of gross aneuploidy in squamous cell 
      carcinomas of the head and neck. Fluorescence in situ hybridization (FISH) with 
      13 centromere-specific DNA probes was applied to 3 diploid and 11 aneuploid 
      tumours with DNA indices ranging between 0.8 and 2.2. Disomic and monosomic cell 
      populations were prevalent findings in DNA-diploid tumours. Polysomies were 
      common in aneuploid tumours. Different degrees of aneusomy for identical 
      chromosomes were recurrent features in aneuploid tumours. FISH signal 
      heterogeneity was identified for all chromosomes. The mean number of aneusomic 
      cell populations identified for DNA-aneuploid tumours ranged between 1.6 for 
      chromosome 17 and 3.1 for chromosome 3. Inconsistencies between FISH and FCM data 
      may indicate that centromere-specific DNA probes identify gains and losses of 
      marker DNA due to complex karyotypic rearrangements rather than absolute changes 
      in chromosome numbers. Overall, there was no evidence of the critical involvement 
      of particular chromosomes in the development of different DNA contents.
FAU - Hemmer, J
AU  - Hemmer J
AD  - Division of Tumor Biology, University of Ulm, Germany. 
      joerg.hemmer@medizin.uni-ulm.de
FAU - Hauser, C
AU  - Hauser C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Anal Cell Pathol
JT  - Analytical cellular pathology : the journal of the European Society for 
      Analytical Cellular Pathology
JID - 8911016
SB  - IM
MH  - Aneuploidy
MH  - Carcinoma, Squamous Cell/*pathology
MH  - Chromosomes/*ultrastructure
MH  - Chromosomes, Human, Pair 17/ultrastructure
MH  - Chromosomes, Human, Pair 3/ultrastructure
MH  - Clone Cells
MH  - *Flow Cytometry
MH  - Head and Neck Neoplasms/*pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
PMC - PMC4618014
EDAT- 2001/02/24 12:00
MHDA- 2001/03/27 10:01
PMCR- 2000/01/01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PHST- 2000/01/01 00:00 [pmc-release]
AID - 235942 [pii]
AID - 10.1155/2000/235942 [doi]
PST - ppublish
SO  - Anal Cell Pathol. 2000;20(4):197-203. doi: 10.1155/2000/235942.