PMID- 11205868
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 68
IP  - 7
DP  - 2001 Jan 5
TI  - Optimization of the technique to isolate fetal hepatocytes, and assessment of 
      their functionality by transplantation.
PG  - 763-72
AB  - In contrast to adult hepatocytes, fetal hepatocytes (FH) are thought to be highly 
      proliferative less immunogenic and more resistant to both cryopreservation and 
      ischemic injury. In the present study, we describe the method for isolation of FH 
      and the relationship between the transplantability of FH into the spleen of 
      analbuminemic rats and expression of albumin mRNA. Rat FH were obtained using the 
      nonperfusion collagenase/DNase digestion method. Nagase analbuminemic rats (NAR), 
      a strain which bears a mutation that determines the impossibility of the normal 
      splicing of the albumin mRNA were used as recipients. The transplanted FH 
      immediately migrated to the liver via portal vein, and anchored there. To assess 
      the functional state of the transplanted cells, one month after transplantation, 
      the expression of the albumin gene was studied in the liver of the recipients.
FAU - Arahuetes, R M
AU  - Arahuetes RM
AD  - Departamento de Biologia Animal II, Facultad de Biologia, Universidad 
      Complutense, Madrid, Spain. rportero@eucmax.sim.ucm.es
FAU - Sierra, E
AU  - Sierra E
FAU - Codesal, J
AU  - Codesal J
FAU - Garcia Barrutia, M S
AU  - Garcia Barrutia MS
FAU - Arza, E
AU  - Arza E
FAU - Cubero, J
AU  - Cubero J
FAU - Ortiz, A
AU  - Ortiz A
FAU - Maganto, P
AU  - Maganto P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Albumins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Messenger)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Albumins/biosynthesis/genetics
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Separation
MH  - Cell Transplantation/*physiology
MH  - Female
MH  - Fetal Tissue Transplantation/*physiology
MH  - Fluorescent Dyes
MH  - Hepatocytes/*transplantation
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Pregnancy
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Wistar
EDAT- 2001/02/24 12:00
MHDA- 2001/03/03 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - S0024320500009796 [pii]
AID - 10.1016/s0024-3205(00)00979-6 [doi]
PST - ppublish
SO  - Life Sci. 2001 Jan 5;68(7):763-72. doi: 10.1016/s0024-3205(00)00979-6.