PMID- 11207388
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 300
IP  - 2
DP  - 2001 Mar 9
TI  - Collaboration of fibronectin matrix and neurotrophin in regulating spontaneous 
      transmitter release at developing neuromuscular synapses in Xenopus cell 
      cultures.
PG  - 115-9
AB  - Integrins mediate cell-extracellular matrix connection and are particularly 
      important during neuronal development. We here investigated the regulation of 
      fibronectin (FN) matrix and neurotrophins on the embryonic synaptic transmission. 
      Spontaneous synaptic currents (SSCs) were recorded from innervated myocytes of 
      1-day-old Xenopus cultures by whole-cell recordings. The SSC increasing action of 
      alpha,beta-methylene adenosine triphosphate was enhanced in neurons grown on FN 
      substratum, which was further potentiated by chronic treatment with brain-derived 
      neurotrophic factor (BDNF). The SSC increasing action of thapsigargin, carbonyl 
      cyanide m-chlorophenylhydrazone and N-methyl-D-aspartate was also markedly 
      potentiated in neurons grown on FN-coated glass coverslips and chronically 
      treated with BDNF. FN matrix or BDNF alone only exerts slight potentiation on the 
      SSC increasing action of these three drugs. Our results suggest that FN matrix 
      can collaborate with neurotrophin in regulating synaptic transmission at 
      developing motoneurons, which may play an important role in the maturation of 
      embryonic neuromuscular junction.
FAU - Fu, W M
AU  - Fu WM
AD  - Pharmacological Institute, College of Medicine, National Taiwan University, 
      Taipei. wenmei@ccms.ntu.edu.tw
FAU - Liou, H H
AU  - Liou HH
FAU - Wang, C L
AU  - Wang CL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fibronectins)
RN  - 0 (Integrins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Uncoupling Agents)
RN  - 555-60-2 (Carbonyl Cyanide m-Chlorophenyl Hydrazone)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - N9YNS0M02X (Acetylcholine)
RN  - NYX13NT29D (alpha,beta-methyleneadenosine 5'-triphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acetylcholine/metabolism
MH  - Adenosine Triphosphate/analogs & derivatives/metabolism/pharmacology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Calcium/metabolism
MH  - Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology
MH  - Cells, Cultured/drug effects/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Extracellular Matrix/drug effects/*metabolism
MH  - Fibronectins/*metabolism/pharmacology
MH  - Integrins/drug effects/metabolism
MH  - Membrane Potentials/drug effects/physiology
MH  - N-Methylaspartate/pharmacology
MH  - Nerve Growth Factors/*metabolism/pharmacology
MH  - Neuromuscular Junction/*drug effects/embryology/*metabolism
MH  - Neurotransmitter Agents/*metabolism
MH  - Neurotrophin 3/pharmacology
MH  - Synaptic Transmission/drug effects/*physiology
MH  - Thapsigargin/pharmacology
MH  - Uncoupling Agents/pharmacology
MH  - Xenopus laevis
EDAT- 2001/02/24 11:00
MHDA- 2001/08/10 10:01
CRDT- 2001/02/24 11:00
PHST- 2001/02/24 11:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/02/24 11:00 [entrez]
AID - S0304394001015671 [pii]
AID - 10.1016/s0304-3940(01)01567-1 [doi]
PST - ppublish
SO  - Neurosci Lett. 2001 Mar 9;300(2):115-9. doi: 10.1016/s0304-3940(01)01567-1.