PMID- 11207556
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20191104
IS  - 1462-5814 (Print)
IS  - 1462-5814 (Linking)
VI  - 1
IP  - 3
DP  - 1999 Nov
TI  - Characterization and intracellular trafficking pattern of vacuoles containing 
      Chlamydia pneumoniae in human epithelial cells.
PG  - 237-47
AB  - Chlamydiae are obligate intracellular pathogens that reside within a 
      membrane-bound vacuole throughout their developmental cycle. In this study, the 
      intraphagosomal pH of Chlamydia pneumoniae (Cpn) was qualitatively assessed, and 
      the intracellular fate of the pathogen-containing vacuole and its interaction 
      with endocytic organelles in human epithelial cells were analysed using 
      conventional immunofluorescence and confocal microscopy. The pH-sensitive probes 
      acridine orange (AO), LysoTracker (LyT) and DAMP did not accumulate in the 
      bacterial inclusion. In addition, exposure of cells to bafilomycin A1(BafA1), a 
      potent acidification inhibitor, did not inhibit or delay chlamydial growth. The 
      chlamydial compartment was not accessible to the fluid-phase tracer Texas Red 
      (TR)-dextran and did not exhibit any level of staining for the late endosomal 
      marker cation-independent mannose-6-phosphate receptor (Ci-M6PR) or for the 
      lysosomal-associated membrane proteins (LAMP-1 and -2) and CD63. In addition, 
      transferrin receptor (TfR)-enriched vesicles were observed close to Cpn vacuoles, 
      potentially indicating a specific translocation of these organelles through the 
      cytoplasm to the vicinity of the vacuole. We conclude that Cpn, like other 
      chlamydial spp., circumvents the host endocytic pathway and inhabits a non-acidic 
      vacuole, which is dissociated from late endosomes and lysosomes, but selectively 
      accumulates early endosomes.
FAU - Al-Younes, H M
AU  - Al-Younes HM
AD  - Max-Planck-Institut fur Infektionsbiologie, Abteilung Molekulare Biologie, 
      Berlin, Germany.
FAU - Rudel, T
AU  - Rudel T
FAU - Meyer, T F
AU  - Meyer TF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Cell Microbiol
JT  - Cellular microbiology
JID - 100883691
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Dextrans)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Macrolides)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Receptors, Transferrin)
RN  - 66974FR9Q1 (Chloramphenicol)
RN  - 88899-55-2 (bafilomycin A1)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Cell Line
MH  - Chlamydophila pneumoniae/growth & development/*pathogenicity
MH  - Chloramphenicol/pharmacology
MH  - Dextrans/metabolism
MH  - *Endocytosis
MH  - Endosomes/metabolism/microbiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Epithelial Cells/metabolism/*microbiology
MH  - HeLa Cells
MH  - Humans
MH  - Hydrogen-Ion Concentration/drug effects
MH  - Inclusion Bodies/drug effects/microbiology
MH  - Lysosomes/metabolism
MH  - *Macrolides
MH  - Membrane Fusion/drug effects
MH  - Microscopy, Confocal
MH  - Phagosomes/metabolism/microbiology
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Receptors, Transferrin/metabolism
MH  - Vacuoles/metabolism/*microbiology
EDAT- 2001/02/24 11:00
MHDA- 2001/07/20 10:01
CRDT- 2001/02/24 11:00
PHST- 2001/02/24 11:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/02/24 11:00 [entrez]
AID - cmi24 [pii]
AID - 10.1046/j.1462-5822.1999.00024.x [doi]
PST - ppublish
SO  - Cell Microbiol. 1999 Nov;1(3):237-47. doi: 10.1046/j.1462-5822.1999.00024.x.