PMID- 11207598
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20191104
IS  - 1462-5814 (Print)
IS  - 1462-5814 (Linking)
VI  - 2
IP  - 5
DP  - 2000 Oct
TI  - Interaction of Bartonella henselae with endothelial cells results in rapid 
      bacterial rRNA synthesis and replication.
PG  - 431-41
AB  - Bartonella henselae is a slow-growing microorganism and the causative pathogen of 
      bacillary angiomatosis in man. Here, we analysed how interaction of B. henselae 
      with endothelial cells might affect bacterial growth. For this purpose, bacterial 
      rRNA production and ribosome content was determined by fluorescence in situ 
      hybridization (FISH) using rRNA-targeted fluorescence-labelled oligonucleotide 
      probes. B. henselae grown on agar plates showed no detectable rRNA content by 
      means of FISH, whereas B. henselae co-cultured with endothelial cells showed a 
      rapid increase of rRNA production within the first 18 h after inoculation. The 
      increased rRNA synthesis was paralleled by a approximately 1000-fold 
      intracellular bacterial replication, whereas bacteria grown on agar base showed 
      only a approximately 10-fold replication within the first 48 h of culture. 
      Pretreatment of host cells with paraformaldehyde prevented adhesion, invasion, 
      intracellular replication and bacterial rRNA synthesis of B. henselae. In 
      contrast, inhibition of host cell protein synthesis by cycloheximide did not 
      affect bacterial adhesion and invasion, but prevented intracellular replication 
      although bacterial rRNA content was increased. Inhibition of actin polymerization 
      by cytochalasin D did not affect adhesion, invasion, increased rRNA content or 
      intracellular replication of B. henselae. These results demonstrate that rRNA 
      synthesis and replication of B. henselae is promoted by viable host cells with 
      intact de novo protein synthesis.
FAU - Kempf, V A
AU  - Kempf VA
AD  - Max von Pettenkofer-Institut fur Hygiene und Medizinische Mikrobiologie, Ludwig 
      Maximilians Universitat Munchen, Munich, Germany. 
      kempf@m3401.mpk.med.uni-muenchen.de
FAU - Schaller, M
AU  - Schaller M
FAU - Behrendt, S
AU  - Behrendt S
FAU - Volkmann, B
AU  - Volkmann B
FAU - Aepfelbacher, M
AU  - Aepfelbacher M
FAU - Cakman, I
AU  - Cakman I
FAU - Autenrieth, I B
AU  - Autenrieth IB
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Cell Microbiol
JT  - Cellular microbiology
JID - 100883691
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Polymers)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 22144-77-0 (Cytochalasin D)
RN  - 98600C0908 (Cycloheximide)
RN  - Y19UC83H8E (paraform)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bartonella henselae/genetics/*pathogenicity
MH  - Cell Line
MH  - Cycloheximide/pharmacology
MH  - Cytochalasin D/pharmacology
MH  - Endothelium/drug effects/*microbiology
MH  - Formaldehyde/pharmacology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Electron, Scanning Transmission
MH  - Nucleic Acid Synthesis Inhibitors/pharmacology
MH  - Polymers/pharmacology
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA, Bacterial/analysis
MH  - RNA, Ribosomal/analysis
EDAT- 2001/02/24 11:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/24 11:00
PHST- 2001/02/24 11:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/24 11:00 [entrez]
AID - cmi72 [pii]
AID - 10.1046/j.1462-5822.2000.00072.x [doi]
PST - ppublish
SO  - Cell Microbiol. 2000 Oct;2(5):431-41. doi: 10.1046/j.1462-5822.2000.00072.x.