PMID- 11207758
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20191210
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 1
IP  - 5
DP  - 1999 Oct
TI  - Development and characterization of a lux-modified 2,4-dichlorophenol-degrading 
      Burkholderia sp. RASC.
PG  - 393-9
AB  - lux-marked biosensors for assessing the toxicity and bioremediation potential of 
      polluted environments may complement traditional chemical techniques. luxCDABE 
      genes were introduced into the chromosome of the 2,4-dichlorophenol 
      (2,4-DCP)-mineralizing bacterium, Burkholderia sp. RASC c2, by biparental mating 
      using the Tn4431 system. Experiments revealed that light output was constitutive 
      and related to cell biomass concentration during exponential growth. The 
      transposon insertion was stable and did not interrupt 2,4-DCP-degradative genes, 
      and expression of luxCDABE did not constitute a metabolic burden to the cell. A 
      bioluminescence response was detectable at sublethal 2,4-DCP concentrations: at < 
      10.26 microg ml(-1), bioluminescence was stimulated (e.g. 218% of control), but 
      at concentrations >60 microg ml(-1) it declined to < 1%. Investigating the effect 
      of [14C]-2,4-DCP concentration on the evolution of 14CO2 revealed that, for 
      initial concentrations of 2.5-25 microg ml(-1), approximately equals 55% of the 
      added 14C was mineralized after 24 h compared with <1% at 50 and 100 microg 
      ml(-1). Inhibition of 2,4-DCP mineralization between 25 and 50 microg ml(-1) 
      corresponded well to the EC50 value (33.83 microg ml(-1)) obtained from 
      bioluminescence inhibition studies. lux-marked RASC c2 may therefore be used as a 
      functionally (i.e. 2,4-DCP degrader) and environmentally relevant biosensor of 
      toxicity and biodegradation inhibition.
FAU - Shaw, L J
AU  - Shaw LJ
AD  - Institute of Terrestrial Ecology, Huntingdon, Cambridgeshire, UK. 
      e.shaw@ite.ac.uk
FAU - Beaton, Y
AU  - Beaton Y
FAU - Glover, L A
AU  - Glover LA
FAU - Killham, K
AU  - Killham K
FAU - Meharg, A A
AU  - Meharg AA
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Luminescent Proteins)
RN  - Q13SKS21MN (2,4-Dinitrophenol)
SB  - IM
MH  - 2,4-Dinitrophenol/*metabolism/toxicity
MH  - Biodegradation, Environmental
MH  - Biosensing Techniques
MH  - Burkholderia/*genetics/growth & development/*metabolism
MH  - Colony Count, Microbial
MH  - DNA Transposable Elements/genetics
MH  - *Luminescent Measurements
MH  - Luminescent Proteins/*genetics/metabolism
MH  - Mutagenesis, Insertional
EDAT- 2001/02/24 11:00
MHDA- 2001/05/05 10:01
CRDT- 2001/02/24 11:00
PHST- 2001/02/24 11:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/02/24 11:00 [entrez]
AID - emi49 [pii]
AID - 10.1046/j.1462-2920.1999.00049.x [doi]
PST - ppublish
SO  - Environ Microbiol. 1999 Oct;1(5):393-9. doi: 10.1046/j.1462-2920.1999.00049.x.