PMID- 11207773
OWN - NLM
STAT- MEDLINE
DCOM- 20010607
LR  - 20191104
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 1
IP  - 6
DP  - 1999 Dec
TI  - Fluorescence in situ hybridization analysis of the prokaryotic community 
      inhabiting crystallizer ponds.
PG  - 517-23
AB  - A fluorescence in situ hybridization (FISH) protocol suitable for the 
      identification of prokaryotes inhabiting hypersaline environments was developed 
      and applied to several crystallizer ponds with salinities above 36% from a 
      multipond solar saltern in Alicante, Spain. Two morphotypes were abundant in 
      these environments: rods and square or square-like prokaryotes that could be 
      affiliated to Bacteria and Archaea, respectively, by FISH with domain-specific 
      probes. FISH with a newly designed probe proved that the archaeal 16S rDNA 
      sequence most frequently recovered from the crystallizers, SPhT, originated from 
      the dominant square-like prokaryotes. These uncultured prokaryotes have the 
      morphology of Walsby's square bacteria. Additionally, FISH with a probe targeted 
      to the genus Haloarcula, members of which are frequently isolated from this 
      environment, indicated that this genus accounts for less than 0.1% of the total 
      prokaryotic community.
FAU - Anton, J
AU  - Anton J
AD  - Departamento de Biotecnologia, Universidad de Alicante, San Vicente del Raspeig, 
      Spain. anton@ua.es
FAU - Llobet-Brossa, E
AU  - Llobet-Brossa E
FAU - Rodriguez-Valera, F
AU  - Rodriguez-Valera F
FAU - Amann, R
AU  - Amann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - *Archaea/genetics/isolation & purification
MH  - DNA Probes
MH  - DNA, Archaeal/analysis/genetics
MH  - DNA, Ribosomal/analysis/genetics
MH  - Haloarcula/isolation & purification
MH  - Haloferax/isolation & purification
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Sodium Chloride
MH  - *Water Microbiology
EDAT- 2001/02/24 11:00
MHDA- 2001/06/08 10:01
CRDT- 2001/02/24 11:00
PHST- 2001/02/24 11:00 [pubmed]
PHST- 2001/06/08 10:01 [medline]
PHST- 2001/02/24 11:00 [entrez]
AID - emi65 [pii]
AID - 10.1046/j.1462-2920.1999.00065.x [doi]
PST - ppublish
SO  - Environ Microbiol. 1999 Dec;1(6):517-23. doi: 10.1046/j.1462-2920.1999.00065.x.