PMID- 11207806
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 13
IP  - 4
DP  - 2001 Feb
TI  - Mismatch between BDNF mRNA and protein expression in the developing visual 
      cortex: the role of visual experience.
PG  - 709-21
AB  - Brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) expression in the 
      rat visual cortex of young and postnatal day 90 (P90) animals is developmentally 
      regulated and influenced by visual experience. In the present paper we compared 
      the expression of BDNF mRNA to the actual changes of BDNF protein occurring 
      during postnatal development and verified whether BDNF protein distribution is 
      controlled by visual activity. To achieve this aim we analysed BDNF mRNA and/or 
      BDNF protein cellular distribution in the rat visual cortex at different 
      postnatal ages by using immunohistochemistry and highly sensitive in situ 
      hybridization. We found that before eye opening (P13), in all cortical layers a 
      large number of visual cortical neurons contain BDNF mRNA with no detectable 
      amount of BDNF protein. At later ages (P23 and P90), the number of 
      BDNF-immunostained cells increases; most neurons are double labelled for BDNF 
      mRNA and protein, and a small group of neurons is labelled only for BDNF protein. 
      The cellular increase of BDNF immunolabelling is blocked in animals deprived of 
      visual experience from birth (dark rearing), with a large population of neurons 
      containing BDNF mRNA but not BDNF protein. This is similar to what is observed 
      before eye opening. Exposure of dark-reared rats to a brief period (2 h) of light 
      restores a good match between BDNF mRNA and BDNF protein cellular expression. We 
      propose that visual experience controls the neuronal content of BDNF mRNA and 
      BDNF protein in developing visual cortex.
FAU - Tropea, D
AU  - Tropea D
AD  - Neuroscience Program, International School for Advanced Studies (SISSA), Via 
      Beirut 2-4, 34014 Trieste, Italy.
FAU - Capsoni, S
AU  - Capsoni S
FAU - Tongiorgi, E
AU  - Tongiorgi E
FAU - Giannotta, S
AU  - Giannotta S
FAU - Cattaneo, A
AU  - Cattaneo A
FAU - Domenici, L
AU  - Domenici L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Parvalbumins)
RN  - 0 (RNA, Messenger)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Cell Count
MH  - Colchicine/administration & dosage/pharmacology
MH  - Darkness
MH  - *Gene Expression Regulation, Developmental
MH  - HeLa Cells/drug effects
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization
MH  - Injections, Intraventricular
MH  - Nerve Tissue Proteins/analysis/*biosynthesis/genetics
MH  - Neuronal Plasticity
MH  - Neurons/drug effects/metabolism
MH  - Parvalbumins/analysis
MH  - Photic Stimulation
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Sensory Deprivation
MH  - Single-Blind Method
MH  - Visual Cortex/growth & development/*metabolism
EDAT- 2001/02/24 11:00
MHDA- 2001/06/29 10:01
CRDT- 2001/02/24 11:00
PHST- 2001/02/24 11:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/02/24 11:00 [entrez]
AID - ejn1436 [pii]
AID - 10.1046/j.0953-816x.2000.01436.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2001 Feb;13(4):709-21. doi: 10.1046/j.0953-816x.2000.01436.x.