PMID- 11208635
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20061115
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 163
IP  - 1
DP  - 2001 Jan
TI  - Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in 
      sarcoidosis.
PG  - 115-21
AB  - In pulmonary sarcoidosis, activated T cells accumulate in the lungs. We 
      hypothesized that the balance between the T-helper type 1 (Th1) cytokines 
      (interferon [IFN]-gamma and interleukin [IL]-2) and Th2 cytokines such as IL-4, 
      IL-5, and IL-10 might explain differences in clinical outcome in pulmonary 
      sarcoidosis, such as why patients of human leukocyte antigen (HLA) type DR17 have 
      a much better prognosis than those of other HLA types. Peripheral blood 
      lymphocytes (PBL) and lymphocytes obtained by bronchoalveolar lavage (BAL) from 
      HLA-typed sarcoidosis patients, as well as PBL from healthy controls, were 
      stimulated in vitro, fixed, and permeabilized with saponin. Thereafter, cells 
      were stained with fluorescence- labeled antibodies specific for intracellular 
      cytokines (IL-2, IL-4, IFN-gamma, and tumor necrosis factor (TNF)-alpha and cell 
      surface markers CD4 and CD8, and were subjected to flow-cytometric analysis. In 
      bronchoalveolar lavage fluid (BALF), there were significantly greater frequencies 
      of T cells positive for IFN-gamma and TNF-alpha than there were among PBL, and 
      significantly fewer cells positive for IL-4, in both the CD4+ and CD8+ subsets. 
      HLA-DR17-positive patients showed a tendency toward a less pronounced Th1 
      response that may be related to their good prognosis. Sarcoidosis patients had 
      higher frequencies of cells positive for IFN-gamma, IL-4, and IL-2 in their blood 
      than did healthy controls, a finding that may reflect the systemic nature of 
      sarcoidosis. A clear Th1 cytokine profile of CD4+ as well as of CD8+ T cells was 
      demonstrated in BALF from sarcoidosis patients. This was most pronounced for CD8+ 
      cells, which may therefore make an important contribution to the inflammatory 
      process in the lungs in pulmonary sarcoidosis.
FAU - Wahlstrom, J
AU  - Wahlstrom J
AD  - Microbiology and Tumour Biology Centre, Karolinska Institutet, and Department of 
      Medicine, Karolinska Hospital, Stockholm, Sweden.
FAU - Katchar, K
AU  - Katchar K
FAU - Wigzell, H
AU  - Wigzell H
FAU - Olerup, O
AU  - Olerup O
FAU - Eklund, A
AU  - Eklund A
FAU - Grunewald, J
AU  - Grunewald J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - CD4-Positive T-Lymphocytes/*chemistry
MH  - CD8-Positive T-Lymphocytes/*chemistry
MH  - Cytokines/*analysis
MH  - Female
MH  - Humans
MH  - Intracellular Fluid/chemistry
MH  - Lung/immunology
MH  - Male
MH  - Middle Aged
MH  - Sarcoidosis/blood/*immunology
EDAT- 2001/02/24 11:00
MHDA- 2001/06/02 10:01
CRDT- 2001/02/24 11:00
PHST- 2001/02/24 11:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2001/02/24 11:00 [entrez]
AID - 10.1164/ajrccm.163.1.9906071 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2001 Jan;163(1):115-21. doi: 
      10.1164/ajrccm.163.1.9906071.