PMID- 11209542
OWN - NLM
STAT- MEDLINE
DCOM- 20010503
LR  - 20191210
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 17
IP  - 10
DP  - 2000 Nov
TI  - Assessing the chromosome copy number in metaphase II oocytes by sequential 
      fluorescence in situ hybridization.
PG  - 596-602
AB  - PURPOSE: Aneuploidy in oocytes is the main cause of failed embryo implantation 
      and of miscarriage. At present, only limited data on the prevalence of aneuploidy 
      in freshly collected human oocytes are available and all studies have been 
      performed with conventional methods for karyotyping. In this feasibility study, 
      multiple-hybridization fluorescence in situ hybridization (FISH) was evaluated as 
      an alternative method to determine the number of chromosomes in oocytes. METHODS: 
      Fifty-two spare oocytes were collected from 23 patients treated with 
      gonadotropins for intrauterine insemination or intracytoplasmic sperm injection. 
      A conventional dual color FISH approach using mixtures of chromosome-specific 
      standard alpha-satellite probes was applied consecutively to the chromosomes of 
      the same metaphase II oocyte. Mixtures of three to six probes were designed in 
      order to allow chromosome identification based on signal color and centromeric 
      index. RESULTS: One hybridization cycle was possible in 52 uninseminated 
      metaphase II oocytes, two hybridizations in 43 oocytes (82.7%), three 
      hybridizations in 30 oocytes (57.6%), four hybridizations in 27 oocytes (51.9%), 
      and five hybridizations in 15 oocytes (28.8%). Altogether, 591 chromosomes could 
      be marked (47.4% of the entire chromosome complement, 11.4 chromosomes per 
      oocyte). The most important single factor contributing to technical failure was 
      loss of the oocyte from the slide. CONCLUSION: This feasibility study 
      demonstrates that multiple-hybridization FISH can be used for the assessment of a 
      larger proportion of the chromosome complement in oocyte as compared to previous 
      studies based on FISH.
FAU - Vollmer, M
AU  - Vollmer M
AD  - University Women's Hospital, Basel, Switzerland.
FAU - Wenzel, F
AU  - Wenzel F
FAU - DeGeyter, C
AU  - DeGeyter C
FAU - Zhang, H
AU  - Zhang H
FAU - Holzgreve, W
AU  - Holzgreve W
FAU - Miny, P
AU  - Miny P
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Gonadotropins)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - *Chromosomes, Human
MH  - Feasibility Studies
MH  - Female
MH  - Fluorescent Dyes
MH  - Gonadotropins/pharmacology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles
MH  - Karyotyping/methods
MH  - Metaphase
MH  - Oocytes/*ultrastructure
PMC - PMC3455449
EDAT- 2001/02/24 12:00
MHDA- 2001/05/05 10:01
PMCR- 2001/11/01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/05/05 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PHST- 2001/11/01 00:00 [pmc-release]
AID - 227875 [pii]
AID - 10.1023/a:1026495527331 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2000 Nov;17(10):596-602. doi: 10.1023/a:1026495527331.