PMID- 11209691
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20191210
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 27
IP  - 11
DP  - 2000
TI  - [Mutagenic analysis on the polyhedrin gene (polh) of Bombyx mori nuclear 
      polyhedrosis virus (BmNPV)].
PG  - 972-81
AB  - In our early studies, the abnormal shape of the polyhedra of Bombyx mori nuclear 
      polyhedrosis virus (BmNPV) induced by chemical mutagens of MMC. 9-AA and EMS 
      occurred, and the genome of the mutated BmNPV obtained from the successive test 
      had some change in the restriction endonuclease partners of EcoRI, BglII and 
      BamHI. The present studies showed that the arrangement of the crystal lattice of 
      the polyhedrin was disorderly, and the SDS-PAGE electropherogram of the 
      polyhedrin depicted distinct change in comparison with control group. The results 
      of sequencing analysis showed that many point mutations with characteristics of 
      the base substitution had occurred at some sites of the BmNPV polh gene in three 
      mutated groups, and these results funther revealed molecular mutagenesis of the 
      mutagens effective to BmNPV. It was not confirmable that the point mutations of 
      polh gene in the mutated BmNPV have relationship to abnormal shape of the 
      polyhedra.
FAU - Cao, Y
AU  - Cao Y
AD  - Department of Sericulture and Fashion Design, South China Agricultural 
      University, Guanzhou 510642, China.
FAU - Xiao, Q X
AU  - Xiao QX
FAU - Huang, Y D
AU  - Huang YD
FAU - Ge, C B
AU  - Ge CB
FAU - Huang, Z R
AU  - Huang ZR
FAU - Liu, L S
AU  - Liu LS
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
RN  - 0 (Occlusion Body Matrix Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (polyhedrin protein, Nucleopolyhedrovirus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bombyx/*virology
MH  - Molecular Sequence Data
MH  - Nucleopolyhedroviruses/*genetics
MH  - Occlusion Body Matrix Proteins
MH  - Point Mutation
MH  - Viral Proteins/*genetics
MH  - Viral Structural Proteins
EDAT- 2001/02/24 12:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2000;27(11):972-81.