PMID- 11211954
OWN - NLM
STAT- MEDLINE
DCOM- 20010322
LR  - 20191104
IS  - 0394-9532 (Print)
IS  - 0394-9532 (Linking)
VI  - 12
IP  - 6
DP  - 2000 Dec
TI  - Age-related modifications in human unstimulated whole saliva: a biochemical 
      study.
PG  - 445-8
AB  - Human whole saliva contains a number of antimicrobial agents, and lysozyme, 
      lactoferrin, secretory IgA and peroxidase are among the best known. Peroxidase 
      catalyzes a reaction involved in the inhibition of bacterial growth and 
      metabolism, and the prevention of hydrogen peroxide accumulation, thus protecting 
      proteins from the action of oxygen and reactive oxygen species (ROS). To better 
      understand the role played by the oxidative stress in the aging process, we 
      studied the relationship between total protein content, peroxidase activity, 
      malondialdehyde (MDA) and nitric oxide (NO) content of human unstimulated whole 
      saliva in 169 healthy subjects subdivided into groups according to age. Our 
      results show a significant decrease in peroxidase activity with age. Moreover, 
      the increase in saliva lipid peroxide levels indicates an enhanced free radical 
      production that may contribute to tissue damage. On the other hand, findings 
      concerning human unstimulated whole saliva NO content showed a significant 
      increase in elderly subjects, suggesting that an enhanced NO production might 
      depend on a stimulation of leukocyte-inducible NO synthase (i-NOS) activity. Our 
      results suggest that during aging the oral tissues may become more susceptible to 
      environmental factors due to a modification in the balance between different 
      antimicrobial agents.
FAU - Salvolini, E
AU  - Salvolini E
AD  - lnstitutes of Biochemistry, University of Ancona, Italy. mazzanti@popcsi.unian.it
FAU - Martarelli, D
AU  - Martarelli D
FAU - Di Giorgio, R
AU  - Di Giorgio R
FAU - Mazzanti, L
AU  - Mazzanti L
FAU - Procaccini, M
AU  - Procaccini M
FAU - Curatola, G
AU  - Curatola G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Aging (Milano)
JT  - Aging (Milan, Italy)
JID - 9102503
RN  - 0 (Lipid Peroxides)
RN  - 0 (Salivary Proteins and Peptides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.- (Peroxidases)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aging/*metabolism
MH  - Child
MH  - Cross-Sectional Studies
MH  - Female
MH  - Humans
MH  - Lipid Peroxides/analysis
MH  - Male
MH  - Malondialdehyde/analysis
MH  - Middle Aged
MH  - Nitric Oxide/analysis
MH  - Peroxidases/analysis
MH  - Reference Values
MH  - Saliva/*chemistry
MH  - Salivary Proteins and Peptides/analysis
EDAT- 2001/02/24 12:00
MHDA- 2001/03/27 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/27 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - 10.1007/BF03339875 [doi]
PST - ppublish
SO  - Aging (Milano). 2000 Dec;12(6):445-8. doi: 10.1007/BF03339875.