PMID- 11213013
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20061115
IS  - 0731-8898 (Print)
IS  - 0731-8898 (Linking)
VI  - 19
IP  - 4
DP  - 2000
TI  - Involvement of specific chromosomes in radiation-induced rearrangements detected 
      by fluorescence in situ hybridization (FISH).
PG  - 319-23
AB  - We investigated the involvement of chromosomes in dicentrics and translocations 
      in human peripheral blood lymphocytes exposed to X-rays in vitro. Chromosomes 2, 
      4, 8, 13, 15, 16, and 22 were analyzed in three cocktails of different 
      combinations using whole chromosome probes (WCP) with the fluorescence in situ 
      hybridization (FISH)-painting technique. The results showed overexpression of 
      chromosomes 2, 8, and 22 in translocation, the majority being of the complete 
      type. Chromosome 4 was underrepresented in translocation formation in both 
      combinations, that is, 2 + 4 + 8 in cocktail I and 4 + 13 + 22 in cocktail II. 
      Its participation in dicentric production was in good agreement with its DNA 
      content in association with 2, 4, and 8, whereas it was underexpressed in the 
      combination of 4 + 13 + 22. DNA-proportional involvement was noticed with 
      chromosomes 13 and 16 in all exchange aberrations. Underexpression of chromosome 
      15 was observed in translocation, which is contradictory to its overexpression in 
      dicentric formation. The participation of chromosome 22 was predominant for both 
      translocations and dicentrics, compared with its DNA content. The overall 
      observation of our study supports the assumption of DNA-proportional distribution 
      of Lucas et al. However, more data are required for chromosomes 4, 8, 15, and 22 
      in combination with other chromosomes.
FAU - Ganguly, B B
AU  - Ganguly BB
AD  - Institute of Radiation Hygiene, Federal Office for Radiation Protection, 
      Oberschleissheim, Germany.
FAU - Romm, H
AU  - Romm H
FAU - Pressl, S
AU  - Pressl S
FAU - Stephan, G
AU  - Stephan G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Environ Pathol Toxicol Oncol
JT  - Journal of environmental pathology, toxicology and oncology : official organ of 
      the International Society for Environmental Toxicology and Cancer
JID - 8501420
SB  - IM
MH  - DNA Damage/*radiation effects
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes
MH  - Radiation Injuries/physiopathology
MH  - Translocation, Genetic/*radiation effects
EDAT- 2001/02/24 12:00
MHDA- 2001/03/03 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PST - ppublish
SO  - J Environ Pathol Toxicol Oncol. 2000;19(4):319-23.