PMID- 11213228
OWN - NLM
STAT- MEDLINE
DCOM- 20010405
LR  - 20190921
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 15
IP  - 11-12
DP  - 2000
TI  - An integrated NAD+-dependent enzyme-functionalized field-effect transistor 
      (ENFET) system: development of a lactate biosensor.
PG  - 671-80
AB  - An integrated NAD+-dependent enzyme field-effect transistor (ENFET) device for 
      the biosensing of lactate is described. The aminosiloxane-functionalized gate 
      interface is modified with pyrroloquinoline quinone (PQQ) that acts as a catalyst 
      for the oxidation of NADH. Synthetic amino-derivative of NAD+ is covalently 
      linked to the PQQ monolayer. An affinity complex formed between the 
      NAD+/PQQ-assembly and the NAD+-cofactor-dependent lactate dehydrogenase (LDH) is 
      crosslinked and yields an integrated biosensor ENFET-device for the analysis of 
      lactate. Biocatalyzed oxidation of lactate generates NADH that is oxidized by PQQ 
      in the presence of Ca2+-ions. The reduced catalyst, PQQH2, is oxidized by O2 in a 
      process that constantly regenerates PQQ at the gate interface. The biocatalyzed 
      formation of NADH and the O2-stimulated regeneration of PQQ yield a steady-state 
      pH gradient between the gate interface and the bulk solution. The changes in the 
      pH of the solution near the gate interface and, consequently, the gate potential 
      are controlled by the substrate (lactate) concentration in the solution. The 
      device reveals the detection limit of 1 x 10(-4) M for lactate and the 
      sensitivity of 24+/-2 mV dec(-1). The response time of the device is as low as 15 
      s.
FAU - Zayats, M
AU  - Zayats M
AD  - Institute of Chemistry, The Hebrew University of Jerusalem, Israel.
FAU - Kharitonov, A B
AU  - Kharitonov AB
FAU - Katz, E
AU  - Katz E
FAU - Buckmann, A F
AU  - Buckmann AF
FAU - Willner, I
AU  - Willner I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Quinolones)
RN  - 0 (Quinones)
RN  - 0U46U6E8UK (NAD)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 72909-34-3 (PQQ Cofactor)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - *Biosensing Techniques
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Lactic Acid/*analysis
MH  - NAD/*metabolism
MH  - PQQ Cofactor
MH  - Quinolones/metabolism
MH  - Quinones/metabolism
EDAT- 2001/02/24 12:00
MHDA- 2001/04/06 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/04/06 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - S0956-5663(00)00120-2 [pii]
AID - 10.1016/s0956-5663(00)00120-2 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2000;15(11-12):671-80. doi: 10.1016/s0956-5663(00)00120-2.