PMID- 11213838
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20190921
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 438
IP  - 1
DP  - 2001 Jan
TI  - Expression of monocyte chemoattractant protein-1 in peritoneal endometriotic 
      cells.
PG  - 70-7
AB  - It is well known that the number of peritoneal macrophages is increased in 
      patients with pelvic endometriosis. We measured the concentration of monocyte 
      chemoattractant protein-1 (MCP-1) using an enzyme-linked immunosorbent assay 
      (ELISA) in the peritoneal fluid of women with and without endometriosis. The 
      expression of MCP-1 in pelvic endometriotic lesions obtained from the peritoneum 
      was also examined using immunohistochemistry and nonradioactive in situ 
      hybridization. The mean concentration of MCP-1 in the peritoneal fluid was 
      significantly higher in the patients with endometriosis (P<0.05). The most 
      significant elevation, compared with non-endometriosis patients, was found in 
      stage I of the disease (P<0.05). However, no statistically significant difference 
      was found among endometriosis stages I, II, III, and IV. Immunohistochemical 
      staining revealed that MCP-1-positive cells were localized in the glandular 
      epithelium of the endometriotic lesions and in the stromal macrophages 
      distributed in those lesions, but normal peritoneal cells were negative. The in 
      situ hybridization method demonstrated expression of MCP-1 mRNA on the 
      endometriotic glandular epithelium and stromal macrophages. These findings 
      suggest that MCP-1 may be involved in the histogenesis and early development of 
      peritoneal endometriosis.
FAU - Yih, S
AU  - Yih S
AD  - Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, 
      Kumamoto City, Japan.
FAU - Katabuchi, H
AU  - Katabuchi H
FAU - Araki, M
AU  - Araki M
FAU - Matsuura, K
AU  - Matsuura K
FAU - Takeya, M
AU  - Takeya M
FAU - Takahashi, K
AU  - Takahashi K
FAU - Okamura, H
AU  - Okamura H
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Ascitic Fluid/*chemistry
MH  - Chemokine CCL2/*analysis/*genetics
MH  - Endometriosis/*metabolism
MH  - Epithelium/chemistry
MH  - Female
MH  - *Gene Expression
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Macrophages, Peritoneal/*chemistry
MH  - RNA, Messenger/analysis
MH  - Stromal Cells/chemistry
EDAT- 2001/02/24 12:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - 10.1007/s004280000263 [doi]
PST - ppublish
SO  - Virchows Arch. 2001 Jan;438(1):70-7. doi: 10.1007/s004280000263.