PMID- 11214800
OWN - NLM
STAT- MEDLINE
DCOM- 20010329
LR  - 20191104
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 2
IP  - 6
DP  - 2000 Dec
TI  - Microenvironments and distribution of nitrifying bacteria in a membrane-bound 
      biofilm.
PG  - 680-6
AB  - The distribution of nitrifying bacteria of the genera Nitrosomonas, Nitrosospira, 
      Nitrobacter and Nitrospira was investigated in a membrane-bound biofilm system 
      with opposed supply of oxygen and ammonium. Gradients of oxygen, pH, nitrite and 
      nitrate were determined by means of microsensors while the nitrifying populations 
      along these gradients were identified and quantified using fluorescence in situ 
      hybridization (FISH) in combination with confocal laser scanning microscopy. The 
      oxic part of the biofilm which was subjected to high ammonium and nitrite 
      concentrations was dominated by Nitrosomonas europaea-like ammonia oxidizers and 
      by members of the genus Nitrobacter. Cell numbers of Nitrosospira sp. were 1-2 
      orders of magnitude lower than those of N. europaea. Nitrospira sp. were 
      virtually absent in this part of the biofilm, whereas they were most abundant at 
      the oxic-anoxic interface. In the totally anoxic part of the biofilm, cell 
      numbers of all nitrifiers were relatively low. These observations support the 
      hypothesis that N. europaea and Nitrobacter sp. can out-compete Nitrosospira and 
      Nitrospira spp. at high substrate and oxygen concentrations. Additionally, they 
      suggest microaerophilic behaviour of yet uncultured Nitrospira sp. as a factor of 
      its environmental competitiveness.
FAU - Schramm, A
AU  - Schramm A
AD  - Max Planck Institute for Marine Microbiology, Bremen, Germany. 
      andreas.schramm@biotek.uni-bayreuth.de
FAU - De Beer, D
AU  - De Beer D
FAU - Gieseke, A
AU  - Gieseke A
FAU - Amann, R
AU  - Amann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 7664-41-7 (Ammonia)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Ammonia/metabolism
MH  - Biofilms/classification/*growth & development
MH  - Bioreactors
MH  - Bradyrhizobiaceae/*growth & development/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Nitrates/*metabolism
MH  - Nitrites/*metabolism
MH  - Nitrobacter/growth & development/metabolism
MH  - Nitrosomonas/growth & development/metabolism
MH  - Oxygen/metabolism
EDAT- 2001/02/24 12:00
MHDA- 2001/04/03 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/04/03 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
AID - 10.1046/j.1462-2920.2000.00150.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2000 Dec;2(6):680-6. doi: 10.1046/j.1462-2920.2000.00150.x.