PMID- 11218751
OWN - NLM
STAT- MEDLINE
DCOM- 20010521
LR  - 20061115
IS  - 0047-1887 (Print)
IS  - 0047-1887 (Linking)
VI  - 54
IP  - 3
DP  - 2000 Nov
TI  - [The dynamics of cytokines and their related substances in skin wounds].
PG  - 346-55
AB  - Molecular-pathological study on cytokines and their related substances in skin 
      wound healing was performed from the view points of forensic pathology; 1. The 
      dynamics of inflammatory cytokines such as interleukin (IL)-1 alpha, IL-1 beta, 
      IL-6 and tumor necrosis factor-alpha (TNF alpha) during murine wound healing was 
      examined at the levels of protein and mRNA. As for the results, the production of 
      those inflammatory cytokines could be considered as one of the local vital 
      reactions after wounding, and it was clarified that those inflammatory cytokines 
      were one of the biological markers for forensic wound age determination. 
      Practically, our immunohistochemical and morphometrical study with human skin 
      wounds with different wound ages suggested that the ratios of IL-1 alpha-positive 
      cells, considerably exceeding 30%, indicated a postinfliction interval of 1 day 
      or less. 2. In mouse skin incision, a significant increase in IL-10 mRNA occurred 
      between 30 and 180 min. The increased expression of IL-10 mRNA could be 
      considered a vital reaction in skin wounds. Furthermore, it was clarified that 
      IL-10 may have an important regulatory role in limiting the extent and duration 
      of inflammatory response during skin wound healing. 3. Fas and Fas ligand (Fas L) 
      were immunohistochemically detectable on macrophages and fibroblasts in skin 
      wound healing process. Morphometrically, the number of cell expressing Fas and/or 
      Fas L reached a peak at 6 days after wounding. Therefore, it is considered that 
      apoptosis through Fas and Fas L may play an important role for reducing the 
      cellularity during proliferative phase of wound healing in mice.
FAU - Ohshima, T
AU  - Ohshima T
AD  - Department of Legal Medicine, Kanazawa University Faculty of Medicine.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Nihon Hoigaku Zasshi
JT  - Nihon hoigaku zasshi = The Japanese journal of legal medicine
JID - 0413715
RN  - 0 (Cytokines)
RN  - 0 (FASLG protein, human)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Fasl protein, mouse)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cytokines/*physiology
MH  - Fas Ligand Protein
MH  - Forensic Medicine
MH  - Interleukin-1/physiology
MH  - Interleukin-10/physiology
MH  - Interleukin-6/physiology
MH  - Membrane Glycoproteins/analysis
MH  - Mice
MH  - RNA, Messenger/analysis
MH  - Skin/*injuries/pathology/physiopathology
MH  - Tumor Necrosis Factor-alpha/physiology
MH  - Wound Healing
EDAT- 2001/02/24 12:00
MHDA- 2001/05/25 10:01
CRDT- 2001/02/24 12:00
PHST- 2001/02/24 12:00 [pubmed]
PHST- 2001/05/25 10:01 [medline]
PHST- 2001/02/24 12:00 [entrez]
PST - ppublish
SO  - Nihon Hoigaku Zasshi. 2000 Nov;54(3):346-55.