PMID- 11219851
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20190513
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 1
IP  - 10
DP  - 1980
TI  - Antibodies to N-hydroxy-2-aminofluorene modified DNA as probes in the study of 
      DNA reacted with derivatives of 2-acetylaminofluorene.
PG  - 807-12
AB  - Antibodies were elicited in rabbits immunized with a mixture of methylated bovine 
      serum albumin and N-hydroxy-2-aminofluorene reacted DNA (DNA-AF). These 
      antibodies were used in competition radioimmunoassays (RIA) with [3H]DNA-AF as 
      radioactive antigen and different N-acetoxy-2-acetylaminofluorene (N-AcO-AAF) and 
      N-hydroxy-2aminofluorene (N-OH-AF) reacted DNAs, deoxyguanosine, dGMP, dGpA and 
      dApG as inhibitors. Based on the results of RIA it is concluded that the binding 
      sites of the two residues, -AF and -AAF, to guanosine in DNA, are essentially the 
      same. Moreover, the same sites seem to be reactive also in the direct reaction of 
      N-AcO-AAF with dGMP. Circular dichroism measurements of modified DNA confirm the 
      RIA results.
FAU - Spodheim-Maurizot, M
AU  - Spodheim-Maurizot M
AD  - Centre de Biophysique Moleculaire, CNRS, Orleans, France.
FAU - Saint-Ruf, G
AU  - Saint-Ruf G
FAU - Leng, M
AU  - Leng M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (2-aminofluorene-DNA complex)
RN  - 0 (Alkylating Agents)
RN  - 0 (Antibodies)
RN  - 0 (DNA Adducts)
RN  - 0 (Deoxyguanine Nucleotides)
RN  - 0 (Fluorenes)
RN  - 6098-44-8 (Acetoxyacetylaminofluorene)
RN  - 61M94X4V24 (N-hydroxy-2-aminofluorene)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Acetoxyacetylaminofluorene/*metabolism/toxicity
MH  - Alkylating Agents/*metabolism/toxicity
MH  - Animals
MH  - *Antibodies
MH  - Binding, Competitive
MH  - Cattle
MH  - Circular Dichroism
MH  - DNA/drug effects/*metabolism
MH  - DNA Adducts/*immunology/metabolism
MH  - Deoxyguanine Nucleotides/metabolism/pharmacology
MH  - Fluorenes/*immunology/*metabolism/toxicity
MH  - Precipitin Tests
MH  - Rabbits
MH  - Radioimmunoassay
EDAT- 1980/01/01 00:00
MHDA- 2001/03/07 10:01
CRDT- 1980/01/01 00:00
PHST- 1980/01/01 00:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 1980/01/01 00:00 [entrez]
AID - 10.1093/carcin/1.10.807 [doi]
PST - ppublish
SO  - Carcinogenesis. 1980;1(10):807-12. doi: 10.1093/carcin/1.10.807.