PMID- 11220307
OWN - NLM
STAT- MEDLINE
DCOM- 20010315
LR  - 20191104
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 2
IP  - 2
DP  - 2000 Apr
TI  - Matching molecular diversity and ecophysiology of benthic cyanobacteria and 
      diatoms in communities along a salinity gradient.
PG  - 217-26
AB  - The phylogenetic diversity of oxygenic phototrophic microorganisms in hypersaline 
      microbial mats and their distribution along a salinity gradient were investigated 
      and compared with the halotolerances of closely related cultivated strains. 
      Segments of 16S rRNA genes from cyanobacteria and diatom plastids were retrieved 
      from mat samples by DNA extraction and polymerase chain reaction (PCR), and 
      subsequently analysed by denaturing gradient gel electrophoresis (DGGE). Sequence 
      analyses of DNA from individual DGGE bands suggested that the majority of these 
      organisms was related to cultivated strains at levels that had previously been 
      demonstrated to correlate with characteristic salinity responses. Proportional 
      abundances of amplified 16S rRNA gene segments from phylogenetic groupings of 
      cyanobacteria and diatoms were estimated by image analysis of DGGE gels and were 
      generally found to correspond to abundances of the respective morphotypes 
      determined by microscopic analyses. The results indicated that diatoms accounted 
      for low proportions of cells throughout, that the cyanobacterium Microcoleus 
      chthonoplastes and close relatives dominated the communities up to a salinity of 
      11% and that, at a salinity of 14%, the most abundant cyanobacteria were related 
      to highly halotolerant cultivated cyanobacteria, such as the recently established 
      phylogenetic clusters of Euhalothece and Halospirulina. Although these organisms 
      in cultures had previously demonstrated their ability to grow with close to 
      optimal rates over a wide range of salinities, their occurrence in the field was 
      restricted to the highest salinities investigated.
FAU - Nubel, U
AU  - Nubel U
AD  - Max Planck Institute for Marine Microbiology, Bremen, Germany. 
      unuebel@montana.edu
FAU - Garcia-Pichel, F
AU  - Garcia-Pichel F
FAU - Clavero, E
AU  - Clavero E
FAU - Muyzer, G
AU  - Muyzer G
LA  - eng
SI  - GENBANK/AJ248289
SI  - GENBANK/AJ248290
SI  - GENBANK/AJ248291
SI  - GENBANK/AJ248292
SI  - GENBANK/AJ248293
SI  - GENBANK/AJ248294
SI  - GENBANK/AJ248295
SI  - GENBANK/AJ248296
SI  - GENBANK/AJ248297
SI  - GENBANK/AJ248298
SI  - GENBANK/AJ248299
SI  - GENBANK/AJ248300
SI  - GENBANK/AJ248301
SI  - GENBANK/AJ248302
SI  - GENBANK/AJ248303
SI  - GENBANK/AJ248304
SI  - GENBANK/AJ248305
SI  - GENBANK/AJ248306
SI  - GENBANK/AJ248307
SI  - GENBANK/AJ248308
SI  - GENBANK/AJ248309
SI  - GENBANK/AJ248310
SI  - GENBANK/AJ248311
SI  - GENBANK/AJ248312
SI  - GENBANK/AJ248313
SI  - GENBANK/AJ248314
SI  - GENBANK/AJ248315
SI  - GENBANK/AJ248316
SI  - GENBANK/AJ248317
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Plant)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Plant)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Cyanobacteria/*classification/genetics
MH  - DNA, Bacterial/analysis
MH  - DNA, Plant/analysis
MH  - Diatoms/*classification/genetics
MH  - *Ecology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Mexico
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Bacterial/analysis
MH  - RNA, Plant/analysis
MH  - RNA, Ribosomal, 16S/analysis
MH  - Sodium Chloride
MH  - *Water Microbiology
EDAT- 2001/02/28 10:00
MHDA- 2001/03/17 10:01
CRDT- 2001/02/28 10:00
PHST- 2001/02/28 10:00 [pubmed]
PHST- 2001/03/17 10:01 [medline]
PHST- 2001/02/28 10:00 [entrez]
AID - 10.1046/j.1462-2920.2000.00094.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2000 Apr;2(2):217-26. doi: 10.1046/j.1462-2920.2000.00094.x.