PMID- 11220620
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20190831
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 52
IP  - 3-4
DP  - 2001
TI  - Haplotype-specific sequence encoding the protein kinase, interferon-inducible 
      double-stranded RNA-dependent activator in the human leukocyte antigen class II 
      region.
PG  - 186-94
AB  - The protein kinase, interferon-inducible double-stranded (ds)RNA-dependent 
      activator (PRKRA) is a dsRNA-binding protein which activates a protein kinase 
      participating in the antiviral activity of interferon. Our previous studies 
      indicated that the nucleotide sequence encoding PRKRA, which appeared to be an 
      intronless gene, was present in PAC HS265J14 containing the human leukocyte 
      antigen (HLA) DR subregion. In this study, we further investigated and 
      characterized the PRKRA gene on the human genome by means of Southern blotting 
      and polymerase chain reaction with homozygous typing cell lines for HLA genes. 
      Results indicated that the presence of PRKRA in the DR subregion was dependent on 
      the DR53 group. Consistently, fluorescence in situ hybridization profiles with 
      PRKRA as a probe showed that the hybridization signal on Chromosome (Chr) 6p21.3 
      was seen only in the samples carrying the DR haplotypes that belonged to the DR53 
      group. Interestingly, another hybridization signal, which was mapped on Chr 
      2q31.2-q32.1, was always detected in the samples examined, i.e., even in the 
      samples negative for the DR53 group. The outcome of a sequence-database homology 
      search further indicated that the PRKRA gene with introns appeared to be present 
      in a recently opened draft-sequence, RP11-65L3 (GenBank accession number 
      AC009948), which is located between D2S335 and D2S2257. Together, the data 
      presented here indicate that the PRKRA gene in the DR subregion is a processed 
      pseudogene (PRKRApsi), which could have been generated only on the DR53 common 
      ancestor's genome, and that the master copy of PRKRApsi is most probably present 
      on Chr 2q31.2-q32.1.
FAU - Chida, S
AU  - Chida S
AD  - Department of Human Genetics, Graduate School of Medicine, University of Tokyo, 
      Japan.
FAU - Hohjoh, H
AU  - Hohjoh H
FAU - Hirai, M
AU  - Hirai M
FAU - Tokunaga, K
AU  - Tokunaga K
LA  - eng
SI  - GENBANK/AC009948
SI  - GENBANK/Z84477
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (DNA Probes)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 2.7.11.1 (eIF-2 Kinase)
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Cell Line
MH  - Chromosomes, Human, Pair 2/*genetics
MH  - DNA Probes/genetics
MH  - Genetic Variation/genetics
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Haplotypes/*genetics
MH  - Homozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns/genetics
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - Polymerase Chain Reaction
MH  - RNA-Binding Proteins/*genetics
MH  - eIF-2 Kinase/*metabolism
EDAT- 2001/02/28 10:00
MHDA- 2001/03/10 10:01
CRDT- 2001/02/28 10:00
PHST- 2001/02/28 10:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2001/02/28 10:00 [entrez]
AID - 10.1007/s002510000270 [doi]
PST - ppublish
SO  - Immunogenetics. 2001;52(3-4):186-94. doi: 10.1007/s002510000270.