PMID- 11223240
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 263
IP  - 1-2
DP  - 2001 Jan 24
TI  - Characterization of a MEN1 ortholog from Drosophila melanogaster.
PG  - 31-8
AB  - Multiple endocrine neoplasia type 1 (MEN1) is a familial cancer syndrome 
      characterized by tumors of the parathyroid, entero-pancreatic neuroendocrine and 
      pituitary tissues and caused by inactivating mutations in the MEN1 gene. Menin, 
      the 610-amino acid nuclear protein encoded by MEN1, binds to the transcription 
      factor JunD and can repress JunD-induced transcription. We report here the 
      identification of a MEN1 ortholog in Drosophila melanogaster, Menin1, that 
      encodes a 763 amino acid protein sharing 46% identity with human menin. 
      Additionally, 69% of the missense mutations and in-frame deletions reported in 
      MEN1 patients appear in amino acid residues that are identical in the Drosophila 
      and human protein, suggesting the importance of the conserved regions. Drosophila 
      Menin1 gene transcripts use alternative polyadenylation sites resulting in 4.3 
      and 5-kb messages. The 4.3-kb transcript appears to be largely maternal, while 
      the 5-kb transcript appears mainly zygotic. The binding of Drosophila menin to 
      human JunD or Drosophila Jun could not be demonstrated by the yeast two-hybrid 
      analysis. The identification of the MEN1 ortholog from Drosophila melanogaster 
      will provide an opportunity to utilize Drosophila genetics to enhance our 
      understanding of the function of human menin.
FAU - Guru, S C
AU  - Guru SC
AD  - Genetics and Molecular Biology Branch, National Human Genome Research Institute, 
      49 Convent Drive, Bethesda, MD 20892, USA.
FAU - Prasad, N B
AU  - Prasad NB
FAU - Shin, E J
AU  - Shin EJ
FAU - Hemavathy, K
AU  - Hemavathy K
FAU - Lu, J
AU  - Lu J
FAU - Ip, Y T
AU  - Ip YT
FAU - Agarwal, S K
AU  - Agarwal SK
FAU - Marx, S J
AU  - Marx SJ
FAU - Spiegel, A M
AU  - Spiegel AM
FAU - Collins, F S
AU  - Collins FS
FAU - Oliver, B
AU  - Oliver B
FAU - Chandrasekharappa, S C
AU  - Chandrasekharappa SC
LA  - eng
SI  - GENBANK/AB023400
SI  - GENBANK/AB040816
SI  - GENBANK/AF109389
SI  - GENBANK/U93236
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - DNA, Complementary/chemistry/genetics
MH  - Drosophila melanogaster/embryology/*genetics/growth & development
MH  - Embryo, Nonmammalian/metabolism
MH  - Embryonic Development
MH  - Exons
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Insect/genetics
MH  - Glutathione Transferase/genetics/metabolism
MH  - Humans
MH  - In Situ Hybridization
MH  - Introns
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics
MH  - *Proto-Oncogene Proteins
MH  - Proto-Oncogene Proteins c-jun/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
MH  - Transcription, Genetic
MH  - Two-Hybrid System Techniques
MH  - Zebrafish
EDAT- 2001/02/27 10:00
MHDA- 2001/04/21 10:01
CRDT- 2001/02/27 10:00
PHST- 2001/02/27 10:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 2001/02/27 10:00 [entrez]
AID - S037811190000562X [pii]
AID - 10.1016/s0378-1119(00)00562-x [doi]
PST - ppublish
SO  - Gene. 2001 Jan 24;263(1-2):31-8. doi: 10.1016/s0378-1119(00)00562-x.