PMID- 11224924
OWN - NLM
STAT- MEDLINE
DCOM- 20010726
LR  - 20220409
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 7
IP  - 1
DP  - 2001 Feb
TI  - Evaluation of metallic and polymeric biomaterial surface energy and surface 
      roughness characteristics for directed cell adhesion.
PG  - 55-71
AB  - Directed cell adhesion remains an important goal of implant and tissue 
      engineering technology. In this study, surface energy and surface roughness were 
      investigated to ascertain which of these properties show more overall influence 
      on biomaterial-cell adhesion and colonization. Jet impingement was used to 
      quantify cellular adhesion strength. Cellular proliferation and extracellular 
      matrix secretion were used to characterize colonization of 3T3MC fibroblasts on: 
      HS25 (a cobalt based implant alloy, ASTM F75), 316L stainless steel, Ti-6Al4V (a 
      titanium implant alloy), commercially pure tantalum (Ta), polytetrafluoroethylene 
      (PTFE), silicone rubber (SR), and high-density polyethylene (HDPE). The metals 
      exhibited a nearly five-fold greater adhesion strength than the polymeric 
      materials tested. Generally, surface energy was proportional to cellular adhesion 
      strength. Only polymeric materials demonstrated significant increased adhesion 
      strength associated with increased surface roughness. Cellular adhesion on metals 
      demonstrated a linear correlation with surface energy. Less than half as much 
      cellular proliferation was detected on polymeric materials compared to the 
      metals. However the polymers tested demonstrated greater than twice the amount of 
      secreted extracellular matrix (ECM) proteins on a per cell basis than the 
      metallic materials. Thus, surface energy may be a more important determinant of 
      cell adhesion and proliferation, and may be more useful than surface roughness 
      for directing cell adhesion and cell colonization onto engineered tissue 
      scaffoldings.
FAU - Hallab, N J
AU  - Hallab NJ
AD  - Department of Orthopedic Surgery, Rush Presbyterian-St. Lukes Medical Center, 
      Chicago, Illinois 60612, USA. nhallab@rush.edu
FAU - Bundy, K J
AU  - Bundy KJ
FAU - O'Connor, K
AU  - O'Connor K
FAU - Moses, R L
AU  - Moses RL
FAU - Jacobs, J J
AU  - Jacobs JJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
RN  - 0 (Biocompatible Materials)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Metals)
RN  - 0 (Polymers)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - *Biocompatible Materials
MH  - Biomedical Engineering/*methods
MH  - *Cell Adhesion
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Extracellular Matrix Proteins/chemistry/metabolism
MH  - Fibroblasts/metabolism/ultrastructure
MH  - Humans
MH  - Metals/*chemistry
MH  - Mice
MH  - Polymers/*chemistry
MH  - Statistics as Topic
MH  - Surface Properties
EDAT- 2001/02/27 10:00
MHDA- 2001/07/28 10:01
CRDT- 2001/02/27 10:00
PHST- 2001/02/27 10:00 [pubmed]
PHST- 2001/07/28 10:01 [medline]
PHST- 2001/02/27 10:00 [entrez]
AID - 10.1089/107632700300003297 [doi]
PST - ppublish
SO  - Tissue Eng. 2001 Feb;7(1):55-71. doi: 10.1089/107632700300003297.