PMID- 11226578
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20200409
IS  - 0168-1702 (Print)
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 74
IP  - 1-2
DP  - 2001 Apr
TI  - Complete genome comparison of porcine reproductive and respiratory syndrome virus 
      parental and attenuated strains.
PG  - 99-110
AB  - Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) 
      genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine 
      strain, were compared and analyzed in order to identify possible sites of 
      attenuation. Of the 44 nucleotide changes, 13 resulted in conservative changes 
      and 18 produced non-conservative changes. The results suggest that key amino 
      acids in ORF1 may contribute to the phenotype of RespPRRS, which includes 
      increased growth rate on MA-104 cells and decreased virulence in swine. The 
      results provide a genetic basis for future manipulation of a PRRSV reverse 
      genetics system.
FAU - Yuan, S
AU  - Yuan S
AD  - Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN 
      55108, USA.
FAU - Mickelson, D
AU  - Mickelson D
FAU - Murtaugh, M P
AU  - Murtaugh MP
FAU - Faaberg, K S
AU  - Faaberg KS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (5' Untranslated Regions)
RN  - 0 (RNA, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
CRI - Virus Res. 2001 Nov 5;79(1-2):189-200. PMID: 11551659
MH  - 5' Untranslated Regions
MH  - Amino Acid Substitution
MH  - Animals
MH  - Base Sequence
MH  - Cell Culture Techniques
MH  - DNA Mutational Analysis
MH  - Genome, Viral
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Open Reading Frames/genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/growth & 
      development/isolation & purification/pathogenicity
MH  - RNA, Viral
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis
MH  - Serial Passage
MH  - Species Specificity
MH  - Swine
MH  - Vaccines, Attenuated/genetics/isolation & purification
MH  - Viral Structural Proteins/genetics
MH  - Viral Vaccines/*genetics/isolation & purification
PMC - PMC7125765
EDAT- 2001/02/28 10:00
MHDA- 2001/07/20 10:01
PMCR- 2001/02/20
CRDT- 2001/02/28 10:00
PHST- 2001/02/28 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/02/28 10:00 [entrez]
PHST- 2001/02/20 00:00 [pmc-release]
AID - S0168170200002501 [pii]
AID - S0168-1702(00)00250-1 [pii]
AID - 10.1016/s0168-1702(00)00250-1 [doi]
PST - ppublish
SO  - Virus Res. 2001 Apr;74(1-2):99-110. doi: 10.1016/s0168-1702(00)00250-1.